| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
Studies on the formation and forming mechanism of the related substance E in potassium clavulanate production by HPLC-MS/MS
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| Autores: |
Zhong,Chuanqing
Cao,Guangxiang
Jin,Xiaoqing
Wang,Fengshan
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| Data: |
2014-06-01
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| Ano: |
2014
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| Palavras-chave: |
Potassium clavulanate/production
Clavulanic acid/formation of the Related substance E
Related substance E/formation
Related substance E/forming mechanism High performance liquid chromatography/biotechnological process
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| Resumo: |
The objective of this study was to investigate the formation and forming mechanism of the related substance E in potassium clavulanate production. The impurity with retention time of 11.1 min in potassium clavulanate final product was confirmed as the related substance E by high performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS).The related substance E analysis during the production of clavulanic acid showed that this impurity could be formed during both the fermentation and purification processes, especially in the later fermentation stage, filtration concentration and back-extraction procedure. Clavulanic acid was the precursor of the related substance E. Studies on its forming mechanism showed that the related substance E was formed by the combination of the imino group of one molecule of clavulanic acid with the carboxyl group of another molecule of clavulanic acid with the opening of β-lactam ring. Results of a multi-factor orthogonal test confirmed that the concentration of clavulanic acid was the dominant factor to accelerate the reaction, while the temperature was another contributing factor. The pH 5.0-6.5 had little impact on the generation of the related substance E.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200391
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/S1984-82502014000200019
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.50 n.2 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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