| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDA
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| Autores: |
Rodrigues,Caroline Danziato
Khalil,Najeh Maissar
Mainardes,Rubiana Mara
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| Data: |
2014-12-01
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| Ano: |
2014
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| Palavras-chave: |
Amphotericin B/determination
Amphotericin B/encapsulation
High performance liquid chromatography/reverse phase/quantitative analysis
Nanoparticles
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| Resumo: |
In this work, we developed and validated an effective reversed-phase HPLC method with photodiode array (PDA) detection for the quantitative analysis of amphotericin B (AmB) in poly(lactide)-poly(ethylene glycol) (PLA-PEG) blend nanoparticles. Chromatographic runs were performed on a reverse phase C18 column using a mobile phase comprising a 9% acetic acid and acetonitrile mixture (40:60, v/v) under isocratic elution with a flow rate of 1 mL/min. AmB was detected at a wavelength of 408 nm. The validation process was performed considering the selectivity, linearity, precision, accuracy, robustness, limit of detection (LOD) and limit of quantitation (LOQ) of the method. A concentration range of 1-20 µg/mL was used to construct a linear calibration curve. The LOQ and LOD were 55 and 18 ng/mL, respectively. The mean recovery of AmB from the samples was 99.92% (relative standard deviation (RSD) = 0.34%, n=9), and the method was robust for changes in the flow rate of the mobile phase (maximum RSD=4.82%). The intra- and inter-assay coefficients of variation were less than 0.59%. The method was successfully used to determine the entrapment efficiency of AmB in PLA-PEG blend nanoparticles.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400859
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/S1984-82502014000400021
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.50 n.4 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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