| Registro completo |
| Provedor de dados: |
Braz. J. Plant Physiol.
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| País: |
Brazil
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| Título: |
Response to chitin in suspension-cultured Citrus aurantium cells
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| Autores: |
Gallão,Maria I.
Cortelazzo,Angelo L.
Fevereiro,Manoel P. S.
Brito,Edy S. de
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| Data: |
2007-03-01
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| Ano: |
2007
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| Palavras-chave: |
Chitin
Citrus aurantium
Elicitation
L-phenylalanine ammonia-lyase
Peroxidase
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| Resumo: |
Morphological changes and enzyme activity in suspension-cultured cells of Citrus aurantium were followed after chitin elicitation. Chitin oligomers presented a rapid effect with a maximum activity after 3 h followed by a gradual decrease to the original levels at 8 h of incubation. Cell walls presented a lignification process and the cytoplasmatic protein became less reactive to anionic stains. In the treated material a reduction in starch grain size, an increase in the number of autophagic vacuoles, deposition of secretion in the pericellular space and a defibrillation of the cell-wall polymers were observed. Chitin oligomers increased peroxidase and L-phenylalanine ammonia-lyase activities. Extracellular peroxidase activity increased from 0.20 U mL-1 after 1 h to 0.45 U mL-1 at 3 h followed by a gradual decrease up to 8 h. The peroxidase isoenzyme pattern revealed a predominance of basic isoenzymes in these cells.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000100008
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| Editor: |
Brazilian Journal of Plant Physiology
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| Relação: |
10.1590/S1677-04202007000100008
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Plant Physiology v.19 n.1 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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