| Registro completo |
| Provedor de dados: |
Braz. J. Vet. Res. Anim. Sci.
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| País: |
Brazil
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| Título: |
Isolation of Mycobacterium spp. in milk from cows suspected or positive to tuberculosis
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| Autores: |
PARDO,Renata Bonini
LANGONI,Hélio
MENDONÇA,Lia Jeanne Pereira
CHI,Kung Dahr
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| Data: |
2001-01-01
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| Ano: |
2001
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| Palavras-chave: |
Tuberculosis
Cattle
Milk
Public health
Mycobacterium
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| Resumo: |
This study was performed considering the public health hazards related to the elimination of mycobacteria through milk of dairy cows suspected or positive for tuberculosis presenting no clinical alterations. A total of 780 milk samples from 52 animals, positive or suspected for tuberculosis, according to Stormont's test, were analysed to detect Mycobacterium spp. The samples consisted of 300 ml/cow, collected in the first milking of the day, during 15 days. Frozen samples were sent to the laboratory, inoculated in Löwenstein-Jensen with reduced glicerol (0.5%) and Stonebrink media and kept under 37ºC for at least 90 days. The genus of each observed colony was initially confirmed by Ziehl-Neelsen and auramin staining methods. The isolation of Mycobacterium spps was confirmed in 78 (10%) samples collected from 19 (36.54%) animals. According to thin layer chromatography, time and temperature growth characteristics and colonies aspects, the 19 animals eliminated: M. avium (5.26%), M. fortuitum (10.52%), M. bovis (5.26%) and Mycobacterium spp. (78.95%).
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000600007
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| Editor: |
Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo
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| Relação: |
10.1590/S1413-95962001000600007
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Veterinary Research and Animal Science v.38 n.6 2001
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| Direitos: |
info:eu-repo/semantics/openAccess
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