| Registro completo |
| Provedor de dados: |
Braz. J. Vet. Res. Anim. Sci.
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| País: |
Brazil
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| Título: |
Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle
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| Autores: |
DAFFNER,José
ABALOS,Pedro
PINOCHET,Lautaro
SCORTTI,Mariela
URCELAY,Santiago
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| Data: |
1999-01-01
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| Ano: |
1999
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| Palavras-chave: |
Brucellosis
Immunodiffusion tests
Vaccination
Cattle
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| Resumo: |
Three soluble antigens were compared by radial immunodiffusion (RID) and agar gel immunodiffusion (AGID) tests: a native haptene (NH) from Brucella melitensis 16M, and a polysaccharide (PS) from B. abortus 1119-3, both obtained by non-hydrolytic methods, and the (O-Chain) polysaccharide extracted also from B. abortus 1119-3 but using an hydrolytic method. Three groups of bovine sera were tested: a) Naturally infected (n = 76); b) Non-infected (n = 130) and c) S-19 vaccinated (n = 61); the sensitivity (Se), the specificity (Sp) and the ability to differentiate vaccinated (ADV) were determined in each group a, b and c respectively. The highest Se in the RID test (84.3%) was achieved by NH; while the three antigens gave 100% Sp. The O-Chain showed 100% ADV in this test. In the AGID test PS antigen showed the best Se (86.6%), and all antigens showed 100% of Sp and ADV. Finally, for its production qualities and efficiency the antigens PS and NH represent a promising alternative for complementary diagnosis of brucellosis.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000300002
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| Editor: |
Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo
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| Relação: |
10.1590/S1413-95961999000300002
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Veterinary Research and Animal Science v.36 n.3 1999
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| Direitos: |
info:eu-repo/semantics/openAccess
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