| Registro completo |
| Provedor de dados: |
Scientia Agricola
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| País: |
Brazil
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| Título: |
Symbiotic efficiency and genetic characteristics of Bradyrhizobium sp. strain UFSM LA 1.3 isolated from Lupinus albescens (H. et Arn)
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| Autores: |
Stroschein,Marcos Roberto Dobler
Eltz,Flávio Luiz Foletto
Antoniolli,Zaida Inês
Lupatini,Manoeli
Vargas,Luciano Kaiser
Giongo,Adriana
Pontelli,Mateus Padoin
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| Data: |
2010-12-01
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| Ano: |
2010
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| Palavras-chave: |
Lupin
Rhizobia
Genotypic approaches
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| Resumo: |
Legume species belonging to the genus Lupinus are annual herb plants. The majority of them are indigenous to the Americas. They are known for nitrogen-fixing symbioses with soil bacteria collectively called rhizobia. The aim of this study was to characterize a rhizobium strain isolated from Lupinus albescens using phenotypic, symbiotic and molecular approaches. Strain UFSM LA 1.3 was tested in vitro according to several parameters: colony size, color and growing rate; acid or alkaline reaction in yeast mannitol media supplemented with bromothymol blue; gum production. Molecular characterization was evaluated by PCR technique using primers BOX A1-R and sequence analysis of the 16S-23S rDNA intergenic region (ITS). ITS sequencing fragments showed genetic similarity with Bradyrhizobium sp. The polymorphism observed by BOX-PCR have shown that strain differs from the reference strain SEMIA 928 and SEMIA 938. The symbiotic efficiency under axenic conditions of UFSM LA 1.3 was 94.6%, without statistical differences compared to the mineral nitrogen fertilized control, to which was applied solution of 400 mg of ammonium nitrate.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000600012
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| Editor: |
São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
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| Relação: |
10.1590/S0103-90162010000600012
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| Formato: |
text/html
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| Fonte: |
Scientia Agricola v.67 n.6 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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