| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Heparin-binding proteins of seminal plasma in Nellore bulls
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| Autores: |
Fernandes,Carlos Eurico
Souza,Fabiana Ferreira de
Souza-Neto,Jayme Augusto
Ribola,Paulo Eduardo Martins
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| Data: |
2009-02-01
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| Ano: |
2009
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| Palavras-chave: |
Bovine
Proteins
Seminal plasma
Bos taurus indicus
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| Resumo: |
The aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa) have not been previously observed and possibly they are specific to Nellore semen.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100048
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/S0103-84782008005000044
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.39 n.1 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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