| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods
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| Autores: |
Reis,Luciana Pimenta
Menezes,Liliane Denize Miranda
Lima,Graciela Kunrath
Santos,Ethiene Luiza de Souza
Dorneles,Elaine Maria Seles
Assis,Débora Cristina Sampaio de
Lage,Andrey Pereira
Cançado,Silvana de Vasconcelos
Figueiredo,Tadeu Chaves de
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| Data: |
2018-01-01
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| Ano: |
2018
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| Palavras-chave: |
C. jejuni
C. coli
Detection methods
VIDAS®30.
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| Resumo: |
ABSTRACT: In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS®30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS®30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of Campylobacter spp. were observed between the chilled and frozen broiler carcasses. In conclusion, real-time PCR was the most sensitive method for the detection of Campylobacter spp. in chilled or frozen broiler carcasses, which were mainly contaminated by C. jejuni.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200451
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/0103-8478cr20161034
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.48 n.2 2018
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| Direitos: |
info:eu-repo/semantics/openAccess
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