| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Callus induction and plant regeneration of tomato (Lycopersicon esculentum cv. IPA 5) via anther culture
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| Autores: |
Brasileiro,Ana Christina Rabello
Willadino,Lilia
Carvalheira,Gianna Griz
Guerra,Marcelo
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| Data: |
1999-12-01
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| Ano: |
1999
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| Palavras-chave: |
Growth regulators
Organogenesis
Tetraploid cells
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| Resumo: |
Different growth regulators combinations were tested on the production of anther callus in tomato cultivar IPA 5. Calli were induced on media supplemented with 1.0mgL-1 gibberellic acid (GA3), 0.05mgL-1 alpha-naphthaleneacetic acid (NAA) plus 0.1mgL-1 6-benzylaminopurine (BAP), or with 1.0mgL-1 BAP plus 1.0mgL-1 NAA. The medium containing 1.0mgL-1 BAP and 1.0mgL-1 NAA produced the highest calli frequency, and promoted plant regeneration by indirect organogenesis, when calli were transferred to 0.01mgL-1 BAP and 0.001mgL-1 NAA. Plants regenerated presented tetraploid cells and rare diploid cells. These tetraploid plants could be used as source for further obtainment of trisomic lines, for the purpose of genic localization studies and protein compounds analysis.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000400008
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/S0103-84781999000400008
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.29 n.4 1999
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| Direitos: |
info:eu-repo/semantics/openAccess
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