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Provedor de dados:  Ciência Rural
País:  Brazil
Título:  Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)
Autores:  Galdiano Júnior,Renato Fernandes
Mantovani,Cibele
Lemos,Eliana Gertrudes de Macedo
Data:  2018-01-01
Ano:  2018
Palavras-chave:  In vitro culture
Native orchid
Glucose
Sucrose
Acclimatization
Resumo:  ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (>90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700203
Editor:  Universidade Federal de Santa Maria
Relação:  10.1590/0103-8478cr20160977
Formato:  text/html
Fonte:  Ciência Rural v.48 n.7 2018
Direitos:  info:eu-repo/semantics/openAccess
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