Registro completo |
Provedor de dados: |
Ciência Rural
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País: |
Brazil
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Título: |
Fetuin: a tool to study the block to polyspermy
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Autores: |
Gonçalves,Paulo Bayard
Graves,Charles
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Data: |
1997-03-01
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Ano: |
1997
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Palavras-chave: |
Murine
Block to polyspermy
Fetuin
Supernumerary sperm
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Resumo: |
The effect of fetuin, an a1-glycoprotein that has protease inhibitor activity, on the biock to potyspermy was determined. Cumulus-oocyte complexes from eCG primed mice were matured in vitro in the presence of 0, 0.01, 0.1, l and 10mg/ml of fetuin in modifica TCM 199. Both in vivo and in vitro matured oocytes were fertilized in the presence of fetuin and incubated for 6 and 24h. Fetuin present in a concentration of 1 mg/ml in the ferlilization but not in the maturation medium was able to induce polyspermy in 52.4% of the eggs. There was a positive relationship between concentration of fetuin in the fertilization medium and the proportion of polyspermic eggs (p<0.05). A significant interaclion between 0.1 and l mg/ml of fetuin during maturation and fertilization was observed (p<0.05). The results of these experiments demonstrate the inhibition of the block to polyspermy using a protease inhibitor during the fertilization.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781997000100022
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Editor: |
Universidade Federal de Santa Maria
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Relação: |
10.1590/S0103-84781997000100022
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Formato: |
text/html
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Fonte: |
Ciência Rural v.27 n.1 1997
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Direitos: |
info:eu-repo/semantics/openAccess
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