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Provedor de dados:  Ciência Rural
País:  Brazil
Título:  Fetuin: a tool to study the block to polyspermy
Autores:  Gonçalves,Paulo Bayard
Graves,Charles
Data:  1997-03-01
Ano:  1997
Palavras-chave:  Murine
Block to polyspermy
Fetuin
Supernumerary sperm
Resumo:  The effect of fetuin, an a1-glycoprotein that has protease inhibitor activity, on the biock to potyspermy was determined. Cumulus-oocyte complexes from eCG primed mice were matured in vitro in the presence of 0, 0.01, 0.1, l and 10mg/ml of fetuin in modifica TCM 199. Both in vivo and in vitro matured oocytes were fertilized in the presence of fetuin and incubated for 6 and 24h. Fetuin present in a concentration of 1 mg/ml in the ferlilization but not in the maturation medium was able to induce polyspermy in 52.4% of the eggs. There was a positive relationship between concentration of fetuin in the fertilization medium and the proportion of polyspermic eggs (p<0.05). A significant interaclion between 0.1 and l mg/ml of fetuin during maturation and fertilization was observed (p<0.05). The results of these experiments demonstrate the inhibition of the block to polyspermy using a protease inhibitor during the fertilization.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781997000100022
Editor:  Universidade Federal de Santa Maria
Relação:  10.1590/S0103-84781997000100022
Formato:  text/html
Fonte:  Ciência Rural v.27 n.1 1997
Direitos:  info:eu-repo/semantics/openAccess
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