| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Polymerase Chain Reaction and blood culture for diagnosis of canine sepsis
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| Autores: |
Silveira,Marcelo Marques da
Cândido,Stéfhano Luis
Santos,Karin Rinaldi dos
Maia,Maerle Oliveira
Souza,Roberto Lopes de
Sousa,Valéria Régia Franco
Almeida,Arleana do Bom Parto Ferreira de
Dutra,Valeria
Nakazato,Luciano
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| Data: |
2018-01-01
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| Ano: |
2018
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| Palavras-chave: |
Blood culture
PCR
Bacteremia
Fungemia
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| Resumo: |
ABSTRACT: Sepsis is characterized by the presence of organ dysfunction secondary to the dysregulated systemic inflammatory response associated with an infection, and has high mortality rates. Traditional diagnostic techniques based on non-microbiological isolation are time-consuming and may delay treatment. Thus, this study aimed to compare bacterial and fungal broad-range polymerase chain reaction (PCR) and blood culture for diagnosis of sepsis in dogs. Blood samples from 88 dogs with suspected sepsis were analyzed by blood culture, and PCR to detect bacterial and fungal DNA. On blood culture, 20 (22.7%) samples tested positive for bacterial isolates; however, none tested positive for fungi. Through PCR analysis, bacterial DNA was detected in 46 (52.3%) animals, whereas fungal DNA was present in one (1.1%) sample. Our results showed that PCR-based testing has important diagnostic value for canine blood infections because it has a shorter turnaround time and higher sensitivity than traditional blood culture.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600452
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/0103-8478cr20170871
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.48 n.6 2018
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| Direitos: |
info:eu-repo/semantics/openAccess
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