| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog
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| Autores: |
Oliveira,Vinícius Vasconcelos Gomes de
Ramos,Rafael Antonio Nascimento
Ramos,Carlos Alberto do Nascimento
Guerra,Neurisvan Ramos
Maia,Frederico Celso Lyra
Alves,Leucio Câmara
Silva Junior,Valdemiro Amaro da
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| Data: |
2017-01-01
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| Ano: |
2017
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| Palavras-chave: |
Leishmaniasis
Dog
Vertical infection
Embryo
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| Resumo: |
ABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absent. The exact time of infection and whether in utero transmission occurs have still not been fully elucidated. This report demonstrates molecular evidence of vertical transmission of L. infantum from a pregnant dog to the embryo. Samples (e.g. vulva, vagina, cervix, uterine body, uterine horn and ovaries) from a female naturally infected by L. infantum and from her embryo were molecularly analyzed by means of qPCR and cPCR followed by DNA sequencing. The gestational age was estimated to be 23±1 day. Through qPCR, the presence of L. infantum DNA was detected in all the samples analyzed (n=7), including the embryo, conversely through cPCR, only four samples (vagina, cervix, uterine body and embryo) were positive. This study demonstrated that transmission of L. infantum from a pregnant dog to the embryo might occur in the early days of pregnancy. In conclusion, this is the first report showing L. infantum infecting a canine embryo.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/0103-8478cr20160553
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.47 n.1 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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