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Provedor de dados:  Ciência Rural
País:  Brazil
Título:  Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
Autores:  Ely,Valessa Lunkes
Gressler,Letícia Trevisan
Sutili,Fernando Jonas
Ribeiro,Márcio Garcia
Costa,Mateus Matiuzzi da
Vargas,Agueda Castagna de
Botton,Sônia de Avila
Data:  2019-01-01
Ano:  2019
Palavras-chave:  Prototheca zopfii
Dairy cattle bovine mastitis
Intramammary infections
Microplate biofilm assay
Virulence factor
Resumo:  ABSTRACT: Prototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofilm-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200451
Editor:  Universidade Federal de Santa Maria
Relação:  10.1590/0103-8478cr20180574
Formato:  text/html
Fonte:  Ciência Rural v.49 n.2 2019
Direitos:  info:eu-repo/semantics/openAccess
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