| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
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| Autores: |
Freitas,Loreta Brandão de
Koehler-Santos,Patrícia
Salzano,Francisco Mauro
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| Data: |
2003-06-01
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| Ano: |
2003
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| Palavras-chave: |
Triticum aestivum L.
Defense proteins
Genetic variability
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| Resumo: |
Leaves from 14 Brazilian genotypes of Triticum aestivum L. were treated with salicylic acid to induce pathogenesis-related (PR) proteins. Inter and intracellular extracts were then obtained and investigated through polyacrilamide gel electrophoresis. Seven bands were observed. Material related to two of them (of 40 and 24 kDa) occurred in intracellular spaces only. DNA from these same genotypes was then amplified through PCR using primers developed from three sequences encoding PR proteins, and compared with previously described sequences. The fragments presented homologies to PR groups 1, 3 (chitinases), and 5 (thaumatin-like). The PR3-like sequence also showed a site characteristic of PRs induced by ethylene and a portion without homology with previous sequences. No variation among genotypes were observed, either for protein extracts or DNA sequences.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000300016
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/S0103-84782003000300016
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.33 n.3 2003
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| Direitos: |
info:eu-repo/semantics/openAccess
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