| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Validation of the multiplex PCR for identification of Brucella spp.
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| Autores: |
Orzil,Lívia de Lima
Preis,Ingred Sales
Almeida,Iassudara Garcia de
Souza,Patrícia Gomes de
Soares Filho,Paulo Martins
Jacinto,Fabrício Barcelos
Fonseca Júnior,Antônio Augusto
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| Data: |
2016-05-01
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| Ano: |
2016
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| Palavras-chave: |
Brucellosis
Molecular identification
Zoonosis
Brucella
Multiplex PCR
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| Resumo: |
ABSTRACT: A multiplex PCR technique for detection of Brucella spp. in samples of bacterial suspension was validated as a complementary tool in the diagnosis of the disease. This technique allows the characterization of the agent without performing biochemical tests, which greatly reduces the time for a final diagnosis, and provides more security for the analyst by reducing the time of exposure to microorganisms. The validation was performed in accordance with the Manual of Diagnostic Tests from OIE (2008) and following the requirements present in the ABNT NBR ISO/IEC 17025:2005. The mPCR validated in this study identified the different species of Brucella ( Brucella abortus , B. suis , B. ovis e B. melitensis ) of bacterial suspension obtained from the slaughterhouse samples, as well as distinguished the biovars (1, 2 e 4; 3b, 5, 6 e 9) of B. abortus in grouped form and differentiated the field strains from vaccine strains, as a quick, useful and less expensive technique in diagnosis of brucellosis in Brazil.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500847
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/0103-8478cr20150065
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.46 n.5 2016
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| Direitos: |
info:eu-repo/semantics/openAccess
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