| Registro completo |
| Provedor de dados: |
Electron. J. Biotechnol.
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| País: |
Chile
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| Título: |
Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
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| Autores: |
Wang,Chuan Tang
Tang,Yue Yi
Wang,Xiu Zhen
Zhang,Shu Wei
Li,Gui Jie
Zhang,Jian Cheng
Yu,Shan Lin
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| Data: |
2011-03-01
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| Ano: |
2011
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| Palavras-chave: |
Chemical mutant
FAD2B
NIRS
Oleate
Peanut
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| Resumo: |
Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.
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| Tipo: |
Journal article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200010
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| Editor: |
Pontificia Universidad Católica de Valparaíso
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| Formato: |
text/html
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| Fonte: |
Electronic Journal of Biotechnology v.14 n.2 2011
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