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Provedor de dados:  Electron. J. Biotechnol.
País:  Chile
Título:  Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings
Autores:  Valenzuela,Eduardo
Hipp,Joseline
Alonso,Claudia
Godoy,Roberto
Alberdi,Miren
Alvarez,Maricel
Saavedra,Isabel
Data:  2008-04-01
Ano:  2008
Palavras-chave:  Cellulose
Ectomycorrhizal fungi
Nothofagus
PH
Phosphatase
Temperature
Resumo:  At present, reforestation has focused on native forests with anthropogenic intervention and eroded soils. There is interest in producing Nothofagus seedlings which can overcome adverse conditions encountered on reforestation sites. It is necessary to find new fungi that can be utilized as mycorrhizal inoculants and that enable the seedlings to increase their tolerance to adverse conditions. Two ectomycorrhizal strains of the fungus Descolea antarctica (D1 and D2) were cultured at different temperatures, pH levels and the activities of amylases, cellulases, and phosphatases were determined. In greenhouse and nursery trials, the growth responses of inoculated Nothofagus obliqua seedlings were evaluated. D1 and D2 exhibited the highest growth rates at 23ºC. Both strains grew at pH levels from 4 to 11. The highest enzymatic activities were registered for amylase (57.2 mg glucose/ml * g of mycelium * hr) and acid phosphatases (58.1 mg p-nitrophenol/ml * g of mycelium * hr) at 37ºC, and acid phospatases (1.720 mg p-nitrophenol/ml * g of mycelium * hr) and alkaline phosphatases (1.360 mg p-nitrophenol/ml * g of mycelium * hr) at pH 4 and pH 11, respectively. We conclude that suitable N. obliqua seedlings for use in reforestation were obtained using D2 as inoculant.
Tipo:  Journal article
Idioma:  Inglês
Identificador:  http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200012
Editor:  Pontificia Universidad Católica de Valparaíso
Formato:  text/html
Fonte:  Electronic Journal of Biotechnology v.11 n.2 2008
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