| Registro completo |
| Provedor de dados: |
Electron. J. Biotechnol.
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| País: |
Chile
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| Título: |
Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)
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| Autores: |
Tang,Yue Yi
Wang,Chuan Tang
Yang,Guan Pin
Feng,Tong
Gao,Hua Yuan
Wang,Xiu Zhen
Chi,Xiao Yuan
Xu,Ya Long
Wu,Qi
Chen,Dian Xu
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| Data: |
2011-09-01
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| Ano: |
2011
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| Palavras-chave: |
Chilling response
Peanut
Real-time quantitative PCR
Suppression subtractive hybridization
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| Resumo: |
To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).
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| Tipo: |
Journal article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500005
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| Editor: |
Pontificia Universidad Católica de Valparaíso
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| Formato: |
text/html
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| Fonte: |
Electronic Journal of Biotechnology v.14 n.5 2011
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