Registro completo |
Provedor de dados: |
Electron. J. Biotechnol.
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País: |
Chile
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Título: |
Isolation of simple sequence repeats from groundnut
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Autores: |
Tang Wang,Chuan
Dao Yang,Xin
Xu Chen,Dian
Lin Yu,Shan
Zhen Liu,Guang
Yi Tang,Yue
Zhi Xu,Jian
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Data: |
2007-07-01
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Ano: |
2007
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Palavras-chave: |
Groundnut
Isolation
Simple sequence repeat
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Resumo: |
SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts
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Tipo: |
Journal article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300015
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Editor: |
Pontificia Universidad Católica de Valparaíso
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Formato: |
text/html
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Fonte: |
Electronic Journal of Biotechnology v.10 n.3 2007
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