| Registro completo |
| Provedor de dados: |
Electron. J. Biotechnol.
|
| País: |
Chile
|
| Título: |
Molecular cloning and expression analysis of 12-oxophytodienoate reductase cDNA by wounding in Solanum tuberosum
|
| Autores: |
Díaz,Mauricio
Polanco,Victor
Ramírez,Ingrid
Peña-Cortés,Hugo
|
| Data: |
2012-01-01
|
| Ano: |
2012
|
| Palavras-chave: |
12-oxophytodienoate reductase
Jasmonic acid
OPDA
Solanum tuberosum
Stress
Wounding
|
| Resumo: |
Jasmonic acid (JA) and 12-oxophytodienoic acid (OPDA) are signal molecules involved in the stress and defense responses in plants. A full-length cDNA clon of OPR3 encoding 12-oxophytodienoate reductase 3, key enzyme involved in the biosynthesis of JA from linolenic acid was obtained from a Solanum tuberosum cDNA library. Sequence analysis showed that OPR3 encoded a polypeptide of 400 amino acids with a predicted molecular mass of 43.9 kDa and pI of 7.72. The deduced amino acid sequence of OPR3 showed high similarities to other 12-oxophytodienoate reductases. A peroxisomal signal sequence indicates OPR3 probable location in peroxisome. Levels of OPR3 mRNA accumulated in potato leaves reaching maximum levels within 1 hr of mechanical wounding. Elevated levels of JA were correlated to expression of the OPR3 gene.
|
| Tipo: |
Journal article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100010
|
| Editor: |
Pontificia Universidad Católica de Valparaíso
|
| Formato: |
text/html
|
| Fonte: |
Electronic Journal of Biotechnology v.15 n.1 2012
|
