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	Provedor de dados Electron. J. Biotechnol. (2) Autor Eissler,Yoanna (2) Kuznar,Juan (2) Conejeros,Pablo (1) Espinoza,Juan C (1) Espinoza,Juan Carlos (1) Garland,David E (1) Jorquera,Esteban (1) Morales,Paz (1) Pavlov,Maria Soledad (1) Tapia,David (1) Torres,Pamela (1) Mais... Palavra-chave Digital image analysis (1) Fluorescence microscope methods (1) IPNV detection (1) Immunofluorescence (1) Infectious pancreatic necrosis virus (IPNV) (1) Mismatch's Tm analysis (1) QPCR (1) SYBR-Green I (1) Mais... Tipo do documento Journal article (2) Ano 2016 (1) 2011 (1) País Chile (2) Idioma Inglês (2)		Registro completo Provedor de dados:  Electron. J. Biotechnol. País:  Chile Título:  Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects Autores:  Pavlov,Maria Soledad Eissler,Yoanna Espinoza,Juan Carlos Garland,David E Kuznar,Juan Data:  2011-01-01 Ano:  2011 Palavras-chave:  Digital image analysis Fluorescence microscope methods Immunofluorescence Infectious pancreatic necrosis virus (IPNV) SYBR-Green I Resumo:  A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody. Tipo:  Journal article Idioma:  Inglês Identificador:  http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011 Editor:  Pontificia Universidad Católica de Valparaíso Formato:  text/html Fonte:  Electronic Journal of Biotechnology v.14 n.1 2011 Fechar

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