| Registro completo |
| Provedor de dados: |
74
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| País: |
Brazil
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| Título: |
Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain
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| Autores: |
Gong,Jie
Xie,Yinjie
Yu,Kun
Yang,Ya'nan
Huang,Huiyang
Ye,Haihui
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| Data: |
2014-06-01
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| Ano: |
2014
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| Palavras-chave: |
Chymotrypsin
Scylla paramamosain
Immune response
Immunocytochemistry
In situ hybridization
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| Resumo: |
Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5'-promoter region which is 1221 bp in length-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization exhibited the CHY-like reactivity presented in resorptive cells of the hepatopancreas. After bacterial challenge with Vibrio alginolyticus, the expression level of SpCHY mRNA was extremely up-regulated at 3 h in hepatopancreas. Our results suggest that SpCHY might play an important role in the mud crab's immune response.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300010
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572014005000007
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.37 n.2 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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