| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Cloning and expression analysis of the chitinase gene Ifu-chit2 from Isaria fumosorosea
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| Autores: |
Meng,Huimin
Wang,Zhangxun
Meng,Xiangyun
Xie,Ling
Huang,Bo
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| Data: |
2015-09-01
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| Ano: |
2015
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| Palavras-chave: |
Isaria fumosorosea
Chitinase
Prokaryotic expression
Quantitative real-time PCR
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| Resumo: |
Entomopathogenic fungi can produce a series of chitinases, some of which function synergistically with proteases and other hydrolytic enzymes to degrade the insect cuticle. In the present study, the chitinase gene Ifu-chit2 from Isaria fumosorosea was investigated. The Ifu-chit2 gene is 1,435-bp long, interrupted by three short introns, and encodes a predicted protein of 423 amino acids with a 22 residue signal peptide. The predicted Ifu-Chit2 protein is highly homologous to Beauveria bassiana chitinase Bbchit2 and belongs to the glycohydrolase family 18. Ifu-Chit2 was expressed in Escherichia coli to verify chitinase activity, and the recombinant enzyme exhibited activity with a colloidal chitin substrate. Furthermore, the expression profiles of Ifu-chit2 were analyzed at different induction times under in vivo conditions. Quantitative real-time PCR analysis revealed that Ifu-chit2 expression peaked at two days post-induction. The expression of chitinase Ifu-chit2 in vivo suggests that the chitinase may play a role in the early stage of pathogenesis.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300381
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-475738320150003
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.38 n.3 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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