| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Sucrose synthase molecular marker associated with sugar content in elite sugarcane progeny
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| Autores: |
Silva,Jorge A. da
Bressiani,Jose A.
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| Data: |
2005-01-01
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| Ano: |
2005
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| Palavras-chave: |
Sucrose
Synthase
Expressed
Sequence
Tag
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| Resumo: |
We describe the development and application of an expressed sequence tag (EST)-derived restriction fragment length polymorphism (RFLP) marker for sugarcane elite genotypes which can be used for quantitative trait loci (QTL) tagging for sugar content. EST-derived RFLP markers for proteins involved in sucrose metabolism have been used in Southern analysis for mapping and gene tagging in elite sugarcane clones. A single dose marker, obtained from a sucrose synthase EST associated with sugar content at the alpha = 0.01 probability level, is presented for sugarcane breeding. Utilization of EST homologues to known genes for generation of molecular markers accelerated the identification of a QTL controlling an important trait-sugar content. Sugarcane bacterial artificial chromosome (BAC) clones hybridizing to the sucrose synthase EST were identified.
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| Tipo: |
Info:eu-repo/semantics/other
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200020
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572005000200020
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.28 n.2 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
|
