| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
A simple procedure for rehybridization of nuclei analyzed previously by fish
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| Autores: |
Carvalho-Salles,Andréa Borduchi
Barril,Nilce
Tajara,Eloiza Helena
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| Data: |
1999-06-01
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| Ano: |
1999
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| Resumo: |
We present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labeled DNA probes. This method makes it possible to reanalyze the same nuclei with different centromeric DNA probes and is useful in cases where a limited number of slides are available.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200006
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47571999000200006
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.22 n.2 1999
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| Direitos: |
info:eu-repo/semantics/openAccess
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