| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes): isolated isoforms and kinetics properties
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| Autores: |
Aquino-Silva,Maria Regina de
Schwantes,Maria Luiza Barcellos
Munin,Flavia Simone
Schwantes,Arno Rudi
Santos,Silvana Pereira dos
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| Data: |
2008-01-01
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| Ano: |
2008
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| Palavras-chave: |
Gene duplication
SMDH
Substrate concentration
Temperature
PH isoforms
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| Resumo: |
Kinetic properties and thermal stabilities of Geophagus brasiliensis skeletal muscle unfractionated malate dehydrogenase (MDH, EC 1.1.1.37) and its isolated isoforms were analyzed to examine a possible sMDH-B* locus duplication in a fixation process influenced by genetic drift. Two optimal pHs were detected: 7.5 for AB1 unfractionated muscle phenotype and its B1 isoform, and 8.0 for AB1B2 unfractionated muscle phenotype, A and B2 isoforms. While G. brasiliensis A isoform could be characterized as thermostable, the duplicated B isoform cannot be assumed as thermolabile. Km values for isolated B2 isoforms were 1.6 times lower than for B1. A duplication event in progress best explains the electrophoretic six-band pattern detected in G. brasiliensis, which would be caused by genetic drift.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200029
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572008000200029
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.31 n.1 suppl.0 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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