| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
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| Autores: |
Santelli,Roberto V.
Navarro-Cattapan,Luci Deise
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| Data: |
2000-03-01
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| Ano: |
2000
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| Resumo: |
A modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572000000100011
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.23 n.1 2000
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| Direitos: |
info:eu-repo/semantics/openAccess
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