| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene
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| Autores: |
Ribon,Andréa de O.B.
Ribeiro,João Batista
Gonçalves,Daniel B.
Queiroz,Marisa V. de
Araújo,Elza F. de
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| Data: |
2009-01-01
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| Ano: |
2009
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| Palavras-chave: |
Penicillium griseoroseum
Polygalacturonase
5' upstream regulatory sequence
Electrophoretic mobility shift assay
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| Resumo: |
Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putative CCAAT box that could justify this expression profile. This element, located 270 bp upstream of the translational start codon, was tested as binding target for regulatory proteins. Analysis of a 170 bp promoter fragment by electrophoretic mobility shift assay (EMSA) with nuclear extracts prepared from mycelia grown in pectin-containing culture medium revealed a high mobility complex that was subsequently confirmed by analyzing it with a double-stranded oligonucleotide spanning the CCAAT motif. A substitution in the core sequence for GTAGG partially abolished the formation of specific complexes, showing the involvement of the CCAAT box in the regulation of the polygalacturonase gene studied.
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| Tipo: |
Info:eu-repo/semantics/other
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100019
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572009005000021
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.32 n.1 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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