Registro completo |
Provedor de dados: |
Genet. Mol. Biol.
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País: |
Brazil
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Título: |
Annexin II mRNA expression in bovine oocytes during follicular development
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Autores: |
Costa,Luis Fabiano Santos da
Machado,Márcia Silveira Netto
Oliveira,João Francisco Coelho de
Zamberlan,Gustavo
Gonçalves,Paulo Bayard Dias
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Data: |
2006-01-01
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Ano: |
2006
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Palavras-chave: |
Bovine oocytes
Annexin II
Retinol
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Resumo: |
We investigated the expression of calcium-dependent phospholipid binding protein annexin-II (Ann-II) messenger RNA (mRNA) during preantral follicle development and in oocytes from antral follicles of different diameters (< 3 mm, 5 to 8 mm and > 8 mm). The action of retinol on Ann-II mRNA expression in mature oocytes was also examined. Only oocytes from secondary preantral follicles expressed Ann-II mRNA and at the germinal vesicle stage expression by oocytes from follicles larger than 8 mm was significantly higher (p < 0.05) compared with oocytes from follicles smaller than 3 mm or between 5 and 8 mm. Ann-II mRNA expression by metaphase II oocytes from follicles larger than 8 mm was significantly higher (p < 0.05) than that from oocytes from follicles smaller than 3 mm, with oocytes from both these size-classes showing similar levels of Ann-II mRNA expression as oocytes recovered from 5-8 mm follicles. In the presence of retinol, Ann-II mRNA expression was higher than when retinol was absent (p < 0.05). Our data indicate that Ann-II mRNA expression is highest in competent oocytes and that retinol increases Ann-II mRNA and may be involved in the regulation of oocyte competence by decreasing the translation and/or degradation of Ann-II mRNA.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200031
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Editor: |
Sociedade Brasileira de Genética
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Relação: |
10.1590/S1415-47572006000200031
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Formato: |
text/html
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Fonte: |
Genetics and Molecular Biology v.29 n.2 2006
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Direitos: |
info:eu-repo/semantics/openAccess
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