| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
|
| País: |
Brazil
|
| Título: |
Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts
|
| Autores: |
Zhang,Shumin
Xiong,Kai
Xie,Zhourui
Nan,Wenting
Liu,Honglin
Chen,Jie
|
| Data: |
2012-01-01
|
| Ano: |
2012
|
| Palavras-chave: |
RNAi
ShRNA
Lentivirus
BLG
Goat
|
| Resumo: |
β-lactoglobulin (BLG), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. Its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. A shRNA targeting BLG mRNA with high interference efficiency was identified, with which lentiviral vectors were used for mediating stable shRNA interference in goat-fetal fibroblast cells. Apart from high efficiency in the knockdown of BLG expression in these cells, lentivector-mediated RNAi manifested stable integration into the goat genome itself. Consequently, an in vitro model for goat BLG-content control was compiled, and a goat-cell line for accompanying transgenetic goat production created.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400020
|
| Editor: |
Sociedade Brasileira de Genética
|
| Relação: |
10.1590/S1415-47572012005000042
|
| Formato: |
text/html
|
| Fonte: |
Genetics and Molecular Biology v.35 n.3 2012
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
