| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
A rapid alternative technique for obtaining silver-positive patterns in chromosomes
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| Autores: |
Kavalco,Karine Frehner
Pazza,Rubens
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| Data: |
2004-01-01
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| Ano: |
2004
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| Palavras-chave: |
Silver staining
Ag-NOR
Microwaves
Chromosome banding
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| Resumo: |
Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results. Microwave irradiation proved to be quite efficient and reliable for this purpose, as it allowed to identify Ag-NORs equivalent to those obtained by the conventional procedure and also to reduce the concentration of the employed reagents, as well as the precipitation of debris on the preparation.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200012
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572004000200012
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.27 n.2 2004
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| Direitos: |
info:eu-repo/semantics/openAccess
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