| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
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| Autores: |
Liang,Qiaoyi
Ding,Jiayi
Zheng,Shu
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| Data: |
2009-01-01
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| Ano: |
2009
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| Palavras-chave: |
Endogenous retrovirus
Noncoding RNA
RT-PCR
Quantitative real-time PCR
Transcription
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| Resumo: |
Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs) identified 50 other LTR homogenous HERV-H elements. Further analysis of these elements revealed that all are structurally incomplete and only five exert transcriptional activity. The results presented here recommend further investigation into a potentially functional HERV-H-related ncRNA.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572009005000082
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.32 n.4 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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