| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
The effect of magnesium on mitotic spindle formation in Schizosaccharomyces pombe
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| Autores: |
Uz,Gulsen
Sarikaya,Aysegul Topal
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| Data: |
2016-09-01
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| Ano: |
2016
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| Palavras-chave: |
Magnesium
Microtubule
Mitosis
Mitotic spindle
Schizosaccharomyces pombe
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| Resumo: |
Abstract Magnesium (Mg2+), an essential ion for cells and biological systems, is involved in a variety of cellular processes, including the formation and breakdown of microtubules. The results of a previous investigation suggested that as cells grow the intracellular Mg2+ concentration falls, thereby stimulating formation of the mitotic spindle. In the present work, we used a Mg2+-deficient Schizosaccharomyces pombe strain GA2, in which two essential membrane Mg2+ transporter genes (homologs of ALR1 and ALR2 in Saccharomyces cerevisae) were deleted, and its parental strain Sp292, to examine the extent to which low Mg2+ concentrations can affect mitotic spindle formation. The two S. pombe strains were transformed with a plasmid carrying a GFP-α2-tubulin construct to fluorescently label microtubules. Using the free Mg2+-specific fluorescent probe mag-fura-2, we confirmed that intracellular free Mg2+ levels were lower in GA2 than in the parental strain. Defects in interphase microtubule organization, a lower percentage of mitotic spindle formation and a reduced mitotic index were also observed in the GA2 strain. Although there was interphase microtubule polymerization, the lower level of mitotic spindle formation in the Mg2+-deficient strain suggested a greater requirement for Mg2+ in this phenomenon than previously thought.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300459
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/1678-4685-GMB-2015-0239
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.39 n.3 2016
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| Direitos: |
info:eu-repo/semantics/openAccess
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