| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Array-CGH testing in spontaneous abortions with normal karyotypes
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| Autores: |
Borovik,Cleide L.
Perez,Ana Beatriz A.
Silva,Luciana R.J. da
Krepischi-Santos,Ana Cristina V.
Costa,Silvia S.
Rosenberg,Carla
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| Data: |
2008-01-01
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| Ano: |
2008
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| Palavras-chave: |
Spontaneous abortion
Chromosomal aberrations
Array-CGH
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| Resumo: |
In about 50% of first trimester spontaneous abortion the cause remains undetermined after standard cytogenetic investigation. We evaluated the usefulness of array-CGH in diagnosing chromosome abnormalities in products of conception from first trimester spontaneous abortions. Cell culture was carried out in short- and long-term cultures of 54 specimens and cytogenetic analysis was successful in 49 of them. Cytogenetic abnormalities (numerical and structural) were detected in 22 (44.89%) specimens. Subsequent, array-CGH based on large insert clones spaced at ~1 Mb intervals over the whole genome was used in 17 cases with normal G-banding karyotype. This revealed chromosome aneuplodies in three additional cases, giving a final total of 51% cases in which an abnormal karyotype was detected. In keeping with other recently published works, this study shows that array-CGH detects abnormalities in a further ~10% of spontaneous abortion specimens considered to be normal using standard cytogenetic methods. As such, array-CGH technique may present a suitable complementary test to cytogenetic analysis in cases with a normal karyotype.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300004
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572008000300004
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.31 n.2 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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