| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
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| Autores: |
Evangelista,Cláudia Carolina Silva
Guidelli,Giovanna Vieira
Borges,Gustavo
Araujo,Thais Fenz
Souza,Tiago Alves Jorge de
Neves,Ubiraci Pereira da Costa
Tunnacliffe,Alan
Pereira,Tiago Campos
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| Data: |
2017-12-01
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| Ano: |
2017
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| Palavras-chave: |
Peroxiredoxin
Kinase
RNAi
Proteostasis
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| Resumo: |
Abstract The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes contribute to anhydrobiosis in P. superbus.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500790
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/1678-4685-gmb-2017-0030
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.40 n.4 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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