| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Satellite DNA sites in four species of the genus Astyanax (Teleostei, Characiformes)
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| Autores: |
Kavalco,Karine Frehner
Pazza,Rubens
Bertollo,Luiz Antonio Carlos
Moreira-Filho,Orlando
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| Data: |
2007-01-01
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| Ano: |
2007
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| Palavras-chave: |
Astyanax
Fluorescence in situ hybridization (FISH)
Heterochromatin
Karyotypic diversity
Satellite DNA
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| Resumo: |
Cytogenetic data about satellite DNA distribution in four Astyanax species (Characidae) from the Paraitinga river, Paraíba do Sul river basin, Brazil, are presented. In order to characterize the constitutive heterochromatin, C-banding, chromomycin A3 and DAPI fluorescence staining, as well as fluorescence in situ hybridization (FISH) with the satellite DNA As-51 probe were performed. A. scabripinnis and A. parahybae presented 2n = 50 and 2n = 48 chromosomes, respectively. The heterochromatin was located in the pericentromeric and terminal regions of many chromosomes, corresponding to GC-positive regions and to the As-51 satellite DNA in terminal regions. A. intermedius and A. giton, both with 2n = 50 chromosomes, showed little heterochromatin, mostly restricted to the terminal and pericentromeric regions of a few chromosomes. No GC-positive regions, neither any correspondence between the scarce heterochromatin of these species and the As-51 satellite DNA was observed. AT-positive blocks were not detected in any of the species studied. Based on these and other available data, the hypothesis that Astyanax represents a polyphyletic group is discussed.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400005
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572007000400005
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.30 n.3 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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