| Registro completo |
| Provedor de dados: |
J. Venom. Anim. Toxins incl. Trop. Dis.
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| País: |
Brazil
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| Título: |
Diagnosis of Giardia infections by PCR-based methods in children of an endemic area
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| Autores: |
David,EB
Coradi,ST
Oliveira-Sequeira,TCG
Ribolla,PEM
Katagiri,S
Guimarães,S
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| Data: |
2011-01-01
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| Ano: |
2011
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| Palavras-chave: |
Giardia duodenalis
Children
Diagnosis
PCR
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| Resumo: |
The present study was designed to estimate the prevalence of Giardia infection in preschool- and school-aged children living in an endemic area. Fecal samples from 573 children were processed by zinc sulfate centrifugal flotation, centrifugal sedimentation (using a commercial device for fecal concentration - TF-Test kit®) and polymerase chain reaction (PCR)-based methods. Of the stool samples assessed, 277 (48.3%) were positive for intestinal parasites and/or commensal protozoa. Centrifugal flotation presented the highest diagnostic sensitivity for Giardia infections. The kappa index revealed that both coproparasitological techniques closely agreed on the Giardia diagnosis (86%) versus satisfactory (72%) and poor (35%) concordances for commensal protozoan and helminth infections, respectively. Concerning Giardia molecular diagnosis, from the 71 microscopy-positive samples, specific amplification of gdh and tpi fragments was noted in 68 (95.7%) and 64 (90%) samples, respectively. Amplification of gdh and tpi genes was observed, respectively, in 95.7% and 90% of microscopy-positive Giardia samples. For 144 microscopy-negative samples, gdh and tpi gene amplification products were obtained from 8.3% and 35.9% samples, respectively. The agreement between these genes was about 40%. The centrifuge-flotation based method was the most suitable means of Giardia diagnosis assessed in the present study by combining accuracy and low cost.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200012
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| Editor: |
Centro de Estudos de Venenos e Animais Peçonhentos
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| Relação: |
10.1590/S1678-91992011000200012
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| Formato: |
text/html
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| Fonte: |
Journal of Venomous Animals and Toxins including Tropical Diseases v.17 n.2 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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