| Registro completo |
| Provedor de dados: |
Neotropical Entomology
|
| País: |
Brazil
|
| Título: |
Characterization of an Argentine isolate of Bacillus thuringiensis similar to the HD-1 strain
|
| Autores: |
Sauka,Diego H
Basurto-Ríos,Regina E
Ibarra,Jorge E
Benintende,Graciela B
|
| Data: |
2010-10-01
|
| Ano: |
2010
|
| Palavras-chave: |
Cryptic gene
Cry1Ac
Cry2Ab
Epinotia aporema
|
| Resumo: |
We report the characterization of an Argentine isolate of Bacillus thuringiensis (INTA TA24-6) similar to the HD-1 strain, which harbors a cryptic cry2Ab gene that apparently is transcribed but not translated into a protein. INTA TA24-6 showed a Rep-PCR pattern identical to the HD-1 strain, a plasmid pattern that resembled that of this strain and cry1 and cry2 genes as HD-1. Screening of cry1 and cry2 genes showed that INTA TA24-6 harbors only cry1Ac and cry2Ab genes. Furthermore, crystalline inclusions of INTA TA24-6 exhibit a bipyramidal shape, typical of Lepidoptera-active B. thuringiensis strains, containing a major protein of ca. 130 kDa toxic to Epinotia aporema Wals. (Lepidoptera: Tortricidae) larvae. Neither the flat-square to cuboidal crystal nor a ca. 65 kDa protein typical of strains expressing Cry2 proteins were detected in INTA TA24-6. In agreement with this information, parasporal crystals of INTA TA24-6 did not show toxicity to Aedes aegypti L. (Diptera:Culicidae) larvae. Gene transcription analyses suggested that the cry2A gene might be cryptic in INTA TA24-6 despite its transcription at different sporulation stages.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000500016
|
| Editor: |
Sociedade Entomológica do Brasil
|
| Relação: |
10.1590/S1519-566X2010000500016
|
| Formato: |
text/html
|
| Fonte: |
Neotropical Entomology v.39 n.5 2010
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
