| Registro completo |
| Provedor de dados: |
PAB
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| País: |
Brazil
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| Título: |
Paternity test in "Mangalarga-Marchador" equines by DNA-fingerprinting
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| Autores: |
ANUNCIAÇÃO,CARLOS EDUARDO
ASTOLFI-FILHO,SPARTACO
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| Data: |
2000-10-01
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| Ano: |
2000
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| Palavras-chave: |
Breeding methods
Molecular cloning
Progeny testing
Horses
Identification
Genetic polymorphism
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| Resumo: |
GC-rich molecular minisatellite probes isolated from the human genome have presented a poor ability for individualization in horses. In this study new DNA sequences were isolated which could be used in paternity tests in horses. Genomic DNA from "Mangalarga-Marchador" horses was treated with restriction enzymes that preferentially digest non-repetitive sequences, so preserving the structure where mini and microsatellites are located. Four clones (S01, S05, S07 and S09) selected from a genomic library screened with a (TG)n oligonucleotide showed similar hybridization profiles generating bands of DNA-fingerprinting type. Using these probes the individualization power obtained was 10-8, which is 10(5)fold higher than that obtained with M13, another GC-rich type probe. All clones were efficient in parentage detection in crossbreedings and presented a 27 bp consensus sequence, GTTTCATTTATTATTCTTTGGAAGAAA, which was repeated 12, 18, 11 and 21 times in clones S01, S05, S07 and S09, respectively.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000001000012
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| Editor: |
Embrapa Secretaria de Pesquisa e Desenvolvimento
Pesquisa Agropecuária Brasileira
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| Relação: |
10.1590/S0100-204X2000001000012
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| Formato: |
text/html
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| Fonte: |
Pesquisa Agropecuária Brasileira v.35 n.10 2000
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| Direitos: |
info:eu-repo/semantics/openAccess
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