| Registro completo |
| Provedor de dados: |
PAB
|
| País: |
Brazil
|
| Título: |
GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange
|
| Autores: |
Azevedo,Fernando Alves de
Mourão Filho,Francisco de Assis Alves
Schinor,Evandro Henrique
Paoli,Luis Gustavo de
Mendes,Beatriz Madalena Januzzi
Harakava,Ricardo
Gabriel,Dean W.
Lee,Richard F.
|
| Data: |
2006-11-01
|
| Ano: |
2006
|
| Palavras-chave: |
Xylella fastidiosa
PAL
Citrus variegated chlorosis
Cultivar resistance
|
| Resumo: |
The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valência' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneration, histochemical analyses using GUS staining revealed that CsPP promoter preferentially, but not exclusively, conferred gene expression in xylem tissues of tobacco. Weaker GUS staining was also detected throughout the petiole region in tobacco and citrus CsPP transgenic plants.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001100008
|
| Editor: |
Embrapa Secretaria de Pesquisa e Desenvolvimento
Pesquisa Agropecuária Brasileira
|
| Relação: |
10.1590/S0100-204X2006001100008
|
| Formato: |
text/html
|
| Fonte: |
Pesquisa Agropecuária Brasileira v.41 n.11 2006
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
