| Registro completo |
| Provedor de dados: |
PAB
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| País: |
Brazil
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| Título: |
Bovine sperm cell motility after incubation in follicular fluid
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| Autores: |
Vargas Júnior,Sergio Farias
Costa,Vitória Gasperin Guazzelli
Oliveira,Fernando Caetano de
Goularte,Karina Lemos
Haas,Cristina Sangoi
Gasperin,Bernardo Garziera
Lucia Júnior,Thomaz
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| Data: |
2018-07-01
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| Ano: |
2018
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| Palavras-chave: |
Infertility
Insemination
Intrafollicular artificial insemination
Spermatozoa
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| Resumo: |
Abstract: The objective of this work was to evaluate sperm cell motility after intrafollicular artificial insemination (IFAI) in vivo or after incubation in follicular fluid in vitro. In the in vivo experiment, IFAI was performed, followed by the recovery of follicular content 1 to 4 hours later, in order to assess sperm motility. In the in vitro experiment, spermatozoa from a pool of commercial frozen-thawed semen were evaluated for their kinetics after incubation for 1 or 3 hours, either pure (pool, control group) or in follicular fluid (FF). A low motility of sperm cells was observed in the FF samples, both in vitro and in vivo. In vitro, the main parameters negatively affected in the sperm cells incubated in FF, compared with the control, were: total motility (TM), progressive motility (PM), curvilinear distance, and straightness, after 1 hour of incubation; and TM, PM, average path velocity, and curvilinear velocity after 3 hours of incubation. The ovarian follicle and follicular fluid do not provide a suitable environment to maintain bovine sperm cell motility.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000700866
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| Editor: |
Embrapa Secretaria de Pesquisa e Desenvolvimento
Pesquisa Agropecuária Brasileira
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| Relação: |
10.1590/s0100-204x2018000700011
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| Formato: |
text/html
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| Fonte: |
Pesquisa Agropecuária Brasileira v.53 n.7 2018
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| Direitos: |
info:eu-repo/semantics/openAccess
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