| Registro completo |
| Provedor de dados: |
Rev. Bras. Ciênc. Avic.
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| País: |
Brazil
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| Título: |
Rotavirus detection and isolation from chickens with or without symptoms
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| Autores: |
Villarreal,LYB
Uliana,G
Valenzuela,C
Chacón,JLV
Saidenberg,ABS
Sanches,AA
Brandão,PE
Jerez,JA
Ferreira,AJP
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| Data: |
2006-09-01
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| Ano: |
2006
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| Palavras-chave: |
Enteric viruses
Rotavirus
Isolation
MA104 cells
Chicken
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| Resumo: |
Rotaviruses have been identified as one of the main etiological agents of diarrhea and enteritis in mammals, including humans, and in avian species. Few studies have been published about enteric viruses in Brazilian poultry, including those related to rotavirus infection. Such studies demonstrate significant occurrence and the importance of enteric viruses in poultry presenting intestinal problems. Enteric viruses are the primary cause of injuries to the gut, allowing other agents, especially bacteria, to attach, to penetrate, and to replicate in the enteric tissue, leading to further damage. The aim of the present study was to detect rotavirus in the intestinal contents of layers and broilers by polyacrylamide gel electrophoresis (PAGE) and virus isolation in MA-104 cell culture. A total of 45.3% of all samples were positive to rotavirus; rotavirus frequencies were 48.7% in samples from flocks with diarrhea, 46.4% in flocks with delayed growth, and 30% in asymptomatic flocks. It was possible to isolate rotavirus in MA-104 cells from the nine rotavirus-positive randomly chosen samples. These results indicate that rotavirus may have an important role in pathogenesis of enteric disease.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000300009
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| Editor: |
Fundação APINCO de Ciência e Tecnologia Avícolas
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| Relação: |
10.1590/S1516-635X2006000300009
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Poultry Science v.8 n.3 2006
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| Direitos: |
info:eu-repo/semantics/openAccess
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