| Registro completo |
| Provedor de dados: |
Rev. Bras. Fisiol. Veg.
|
| País: |
Brazil
|
| Título: |
Isolation and partial purification of beta-galactosidases from cotyledons of two cowpea cultivars
|
| Autores: |
ENÉAS-FILHO,JOAQUIM
BARBOSA,GISLAINY KARLA DA COSTA
SUDÉRIO,FABRÍCIO BONFIM
PRISCO,JOSÉ TARQUÍNIO
GOMES-FILHO,ENÉAS
|
| Data: |
2001-01-01
|
| Ano: |
2001
|
| Palavras-chave: |
Cotyledons
Isozymes
Vigna unguiculata
Vita 3 and Vita 5 cultivars
Salt tolerance
|
| Resumo: |
Three isoforms of beta-galactosidases were isolated and partially purified from the cotyledons of quiescent seeds of Vita 3 and Vita 5 cowpea [Vigna unguiculata (L.) Walp.] cultivars differing in water and salt stress tolerance. The purification procedure consisted of ammonium sulfate fractionation, acid precipitation, ion exchange chromatography through DEAE-sephadex and affinity chromatography through Lactosyl-sepharose columns. The three isoforms isolated from the two cultivars showed the same chromatographic patterns, same optimum of temperature for enzyme activity assay (60ºC), identical thermal stability up to 50°C, and similar pH optima (3-4). However, they differed from each other in sensitivity towards metal ions and certain chemical agents presents in the assay medium. The results have shown that the observed differences in beta-galactosidases from the cotyledons of quiescent seeds were not sufficient to relate them to stress tolerance.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000300001
|
| Editor: |
Sociedade Brasileira de Fisiologia Vegetal
|
| Relação: |
10.1590/S0103-31312001000300001
|
| Formato: |
text/html
|
| Fonte: |
Revista Brasileira de Fisiologia Vegetal v.13 n.3 2001
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
