Registro completo |
Provedor de dados: |
Rev. bras. sementes
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País: |
Brazil
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Título: |
Action of gibberellic acid (GA3) on dormancy and activity of alpha-amylase in rice seeds
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Autores: |
Vieira,Antônio Rodrigues
Vieira,Maria das Graças Guimarães Carvalho
Fraga,Antônio C.
Oliveira,João Almir
Santos,Custódio D. dos
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Data: |
2002-01-01
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Ano: |
2002
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Palavras-chave: |
Rice seeds
Dormancy
Germination
Gibberellic acid (GA3)
Alpha-amylase
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Resumo: |
To evaluate the effectiveness of gibberellic acid (GA3) in breaking rice seed dormancy and the use of alpha-amylase enzyme activity as an indicator of the dormancy level, seed from the intensively dormant irrigated cultivar Urucuia were used. The seeds were submitted to a pre-drying process in a forced air circulation chamber under 40ºC during 7 days and submersed in 30 mL of GA3 solution under 0, 10, 30 and 60 mg/L H2O concentrations, during 2, 24 and 36 hours. After the treatments, the alpha-amylase activity was determined by using the polyacrilamide electrophoresis and spectrophotometry. At the same time, the germination test was made. The results indicated a gain in germination and in alpha-amylase activity in higher concentrations and soaking time of seeds in GA3. These observations support the conclusion that soaking seed in 60 mg GA3/L during 36 hours can be used as a quick and efficient treatment in breaking rice seed dormancy and is equivalent to the forced air circulation chamber at 40ºC during 7 days. The alpha-amylase enzyme activity proved to be as an efficient marker of the seeds dormancy level.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000200008
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Editor: |
Associação Brasileira de Tecnologia de Sementes
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Formato: |
text/html
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Fonte: |
Revista Brasileira de Sementes v.24 n.2 2002
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Direitos: |
info:eu-repo/semantics/openAccess
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