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Registros recuperados: 34
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Biological effects of the "Erika" oil spill on the common mussel (Mytilus edulis) ArchiMer
Bocquene, Gilles; Chantereau, Sebastien; Clerendeau, Christelle; Beausir, Emilie; Menard, Dominique; Raffin, Bernard; Minier, Christophe; Burgeot, Thierry; Leszkowicz, Annie Pfohl; Narbonne, Jean-françois.
A 3-year survey was made of several biological markers in mussels (Mytilus edulis) exposed in situ to the oil that came ashore after the wreck of the "Erika" tanker on the Brittany (France) coast in December 1999. The mussel response was assessed using a set of 7 biomarkers, most of them related to the metabolism of organic contaminants. After a series of validation tests, data was evaluated for only 5 biomarkers: acety1cholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT), malondialdehyde (MDA) and deoxyribonucleic acid (DNA) adducts. No significant reductions in GST or CAT levels were observed, levels of DNA adducts and MDA were high during the 6 months immediately following the accident and levels of AChE were significantly lower during...
Tipo: Text Palavras-chave: Mytilus edulis; Mussel; Biomarkers; Monitoring; Survey; PAH; Polycyclic aromatic hydrocarbon; Oil spill.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-403.pdf
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Contamination des milieux aquatiques par les substances pharmaceutiques et cosmétiques - Etat des lieux et perspectives ArchiMer
Collette-bregand, Maude; James, Alice; Munshy, Catherine; Bocquene, Gilles.
This document reports a survey of the aquatic contamination by cosmetics and medicinal products for human and veterinary uses. The contamination of the continental, estuary and coastal waters by these substances gives rise to questions on environmental risk assessment and management. Among the pharmaceutical classes involved, substances such as anti-inflammatory drugs, steroid hormones, antibiotics and hypolipemiants are of particular concern. Present regulations do not take into account the medical effluents in the environment. A significant part of these substances is not destroyed in water treatment plants whereas veterinary substances are directly spread in the environment. Some molecules occur in particularly high concentrations as shown for...
Tipo: Text Palavras-chave: Médicaments; Produits pharmaceutiques; Vétérinaires; Cosmétiques; Émergents; Évaluation du risque environnemental; Risque chimique; Milieu marin; Pharmaceuticals; Ecotoxicity; Environmental risk assessment; Prioritization methods; Endocrine disruptors; Sewage; Sea water.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00066/17773/15295.pdf
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Effets létaux et sublétaux à long terme d'un nonylphénol polyéthoxyle, le NP 5 sur la civelle ArchiMer
Bocquene, Gilles; Miossec, Laurence.
Studying different concentrations, we have determined LT 50 of a non-ionic surfactant on elver eel: the polyoxyethylated nonylphenol NP 5. Then we studied sorne sublethal effects on fish behaviour and histological effects on gills, during 21 days. We demonstrated primary effects on gills structure and we believe in an impairing of the central nervous system.
Tipo: Text Palavras-chave: Anguille; Tensio-actifs; Effets subletaux; Branchies; Eel; Surfactant; Sublethal effects; Behaviour; Gills.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00156/26705/24780.pdf
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Effets endocriniens des contaminants en milieu marin ArchiMer
Melis, Blandine; Bocquene, Gilles; Raffin, Bernard.
This report presents the state of art of endocrine disruption aspects in ecotoxicology. Some contaminants of the aquatic environment are able to mimic female fish hormones and thereby cause inappropriate feminisation of male fish. This feminisation involves artificial induction and emergence of ovotestis in male (oestrogenic disruption). Natural and synthetic oestrogenic hormones and several contaminants (alkylphenols, PCBs, phtalates and some pesticides) are already known to cause widespread feminisation of male. Two different types of analysis can be performed to detect oestrogenics effects. Immunochemical analysis of plasma (Enzyme-linked immunosorbent assay ELISA and radioimmuno assay RIA) shows that xenoestrogens induce vitellogenin and protein zona...
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Ano: 1999 URL: http://archimer.ifremer.fr/doc/00076/18758/16328.pdf
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The dragonet Callionymus lyra, a target species used for evaluation of biological effects of chemical contaminants on French coasts ArchiMer
Burgeot, Thierry; Bocquene, Gilles; Truquet, Philippe; Le Dean, Loic; Poulard, Jean-charles; Dorel, Didier; Souplet, Arnauld; Galgani, Francois.
An oceanographic cruise in 1991 along the English Channel and Atlantic coasts of France indicated the value of dragonet Callionymus lyra Linnaeus, 1758 as a target species for monitoring biological effects. Ethoxyresorufin-O-deethylase measurements in this species provided a first assessment of the effects of hydrocarbons (PCB, PAH, dioxins) on the marine environment. The specific behavior of dragonet was defined by biochemical characterization. Biological data were obtained during 3 additional oceanographic cruises to study changes in the abundance and distribution of the species in the English Channel and the Bay of Biscay. This first assessment, carried out in the context of the National Observation Network, indicated that pollutants do not have...
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Ano: 1993 URL: http://archimer.ifremer.fr/doc/00091/20243/17895.pdf
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Link between exposure of fish (Solea solea) to PAHs and metabolites: Application to the "Erika" oil spill ArchiMer
Budzinski, Hélène; Mazeas, Olivier; Tronczynski, Jacek; Desaunay, Yves; Bocquene, Gilles; Claireaux, Guy.
An analytical method consisting in enzymatic deconjugation, solid phase extraction and purification, and gas chromatography/mass spectrometry analysis after derivatization was used in this study to quantify Polycyclic Aromatic Hydrocarbon (PAH) metabolites in the bile of fish. The method has been applied in a laboratory experiment studying the fate of pyrene in basin containing soles. This study has allowed the identification of 1-hydroxypyrene as the single metabolite in bile after enzymatic deconjugation. In a second time, 1-hydroxypyrene has been used as a biomarker of exposure in the case of the "Erika" oil spill. This biomonitoring was successful in demonstrating the exposure of juvenile soles to PAHs present in the "Erika" fuel oil.
Tipo: Text Palavras-chave: Gas chromatography mass spectrometry; Fish; PAH metabolites.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-404.pdf
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Influence de la Chloration sur les Premiers Stades Larvaires de Palaemon serratus (Pennant). ArchiMer
Lassus, Patrick; Maggi, Pierre; Le Baut, Claire; Bardouil, Michèle; Bocquene, Gilles; Le Dean, Loïc; Truquet, Philippe.
In order to estimate the effects of chlorine used in cooling systems of electric power plants on zooplankton, some experiments have been realized with the four first larval stages of the prawn (P. serratus Pennant). Chlorine injection was performed by constant adding at 16 and 20 degree C with times exposures of 5, 15 and 25 minutes. If there is no detectable variations in sensitivity of different stages, toxic effects of oxydants issued from chlorination can be observed (for both the two acclimatation temperatures) at 15 minutes exposure with a LD 50 : 32 mg/l of chlorine.
Tipo: Text Palavras-chave: Palaemon serratus; Pollution effects; Larval development; Chlorine; Toxicity.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/publication-1886.pdf
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Contamination of some aquatic species with the organochlorine pesticide chlordecone in Martinique ArchiMer
Coat, Sophie; Bocquene, Gilles; Godard, Eric.
Martinique is a French overseas department whose economy relies heavily on agriculture. Organochlorine pesticides, mainly chlordecone, were used for banana cultivation to eradicate banana weevil over a period of 40 years. Chlordecone is chemically stable,and has a strong affinity for fatty tissues. It is therefore able to bioaccumulate in animals and thereby represent a threat to ecosystems and man. Soils from banana plantations in Martinique are heavily contaminated with chlordecone. Possible transfer of these molecules from agricultural watersheds to the aquatic environment and the organisms that live in it is feared. The hypothesis that ecosystems of Martinique might be highly contaminated with this organochlorine pesticide was investigated. Chlordecone...
Tipo: Text Palavras-chave: W Atlantic; Caribbean Islands; Food web; Aquatic organisms; Chlordecone; Contamination; Pesticides.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1662.pdf
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Juin 1986 : Distribution de Dinophysis au large de la Bretagne sud ArchiMer
Lassus, Patrick; Le Dean, Loic; Bocquene, Gilles; Bardouil, Michele; Truquet, Philippe; Truquet, Isabelle; Larrazabal, M.e..
During EIGAS échointegration cruise, hydrological and phytoplanktonic sampling operations have been carried out along off shore and coastal South Brittany area, from June 8 th to 20 - 1986. Dinophysis cells densities have been evaluated at 3 depths and for 32 statlons between Penmarc'h and Belle Ile, and positive correlation with water stability index have been pointed out; high cells densities area have been detected in the South of Loire estuary
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Ano: 1986 URL: http://archimer.ifremer.fr/doc/00119/23000/20830.pdf
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Chlordecone in the marine environment around the French West Indies: from measurement to pollution management decisions ArchiMer
Bertrand, Jacques; Bodiguel, Xavier; Abarnou, Alain; Reynal, Lionel; Bocquene, Gilles.
Chlordecone is a very persistent insecticide used in banana plantations of the French West Indies between 1972 and 1993. Chlordecone residues were found in inland water, in agricultural and freshwater products, and in marine organisms. This pollution has become of great concern in 2007. Therefore, a governmental action Plan was launched to better assess the pollution and to improve the consumer’s protection. Within this plan, 1048 samples from 69 different species of marine fishes and crustaceans were collected all around both the Guadeloupe and the Martinique Islands and analyzed. The results confirm the presence of chlordecone in marine organisms, with highly variable concentrations (from the detection limit to 1000 μg.kg-1). In 17.9 % of the samples,...
Tipo: Text Palavras-chave: Chlordecone (Kepone); French West Indies; Fishes; Crustaceans; Organochlorinated contaminant; Risk assessment; Pollution management.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12511/9361.pdf
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L'acétylcholinestérase, marqueur de neurotoxicité. Application à la surveillance des effets biologiques des polluants chez les organismes marins ArchiMer
Bocquene, Gilles.
Les cholinestérases et particulièrement l'acétylcholinestérase (AChE) constituent la cible privilégiée de nombreuses molécules neurotoxiques notamment des insecticides organophosphorés (OP) et carbamates. La sensibilité des cholinestérases à ces inhibiteurs est aussi complexe que le polymorphisme de ces enzymes. L'utilisation des niveaux d'activité des cholinestérases comme marqueur d'exposition aux neurotoxiques dans le milieu marin implique donc une connaissance approfondie du polymorphisme de i'enzyme, de Ja sensibilité des formes AChE identifiées et des contaminants neurotoxiques susceptibles de provoquer des variations dans l'activité de cette enzyme chez les organismes marins. Aprés une synthèse des connaissances sur le polymorphisme des...
Tipo: Text Palavras-chave: Biomarqueur; Cholinestérases; Pollution marine; Huître; Organophosphorés; Carbamates.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00443/55435/56950.pdf
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Monitoring of pollutant biochemical effects on marine organisms of the french coasts ArchiMer
Galgani, Francois; Bocquene, Gilles; Truquet, Philippe; Burgeot, Thierry; Chiffoleau, Jean-francois; Claisse, Didier.
The establishment of a network to assess pollutant effects is a current concern of international bodies such as the North Sea Task Force, the International Council for the Exploration of the Sea and the Intergovernmental Oceanographic Commission. With respect to the French coasts, three major considerations have been defined : the choice of monitoring areas ; the determination of a target species according to different criteria such as wide distribution, limited migration and contact with pollutant-rich sediments; and the selection of suitable parameters. This paper concerns the strategy adopted for the French coast. Ethoxyresorufin-O-deethylase, a specific cytochrome P450-dependent monooxygenase, measured by a simple microplate assay, was selected as an...
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Ano: 1992 URL: http://archimer.ifremer.fr/doc/00100/21169/18787.pdf
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Réponse des organismes marins aux polluants bases biologiques et moléculaires ArchiMer
Vincent, Francoise; Cachot, Jerome; Galgani, Francois; Bocquene, Gilles.
Analyse moléculaire de la mutagénèse Pour prédire les effets à long tenne de l'exposition aux polluants, la recherche s'oriente sur le développement, la validation et l'application de biomarqueurs de mutagénèse. Les mutations sur les gènes associés au développement tumoral peuvent être détectés dans des stades précoces du cancer, c'est le cas des gènes de la famille Ras et du gène supprésseur de tumeur P53. Les travaux ont porté sur l'étude de l'activation du protooncogène Ras chez les poissons, pour savoir si elle pouvait être corrélée avec l'apparition de tumeurs et à la présence de contaminants cancérigènes. Les exons 1 et 2 du gène Ras de Callionymus lira et de Mullus barbutus ont été amplifiés, clonés et séquencés pour détecter des mutations liées à...
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Ano: 1994 URL: http://archimer.ifremer.fr/doc/00132/24307/22305.pdf
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Etude l'écotoxicité des sédiments dragues - Documents de synthèse et annexes - Projet du groupe GEODE ArchiMer
Quiniou, Francoise; Blanchard, Michel; Bocquene, Gilles; Burgeot, Thierry; Giboire, Louis; His, Edouard; Le Du, Anne.
La présente étude a pour objectif de sélectionner des méthodes de laboratoire et de terrain les mieux appropriées pour l'évaluation de l'écotoxicité de sédiments dragués : indicateurs biochimiques d'exposition (EROD et AChE), tests de toxicité sublétale (micro-organismes, algues, embryons de bivalve et extrait enzymatiques de poisson), indices physiologiques de bivalve (respiration, excrétion, mouvements valvaires). Une synthèse des résultats est présentée accompagnée, en annexes, des rapports des différents participants à l'étude.
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Ano: 1994 URL: http://archimer.ifremer.fr/doc/00104/21573/19153.pdf
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Influence des rejets de l'usine "Thann et Mulhouse" sur les organismes marins - Mesures de pH "in situ" et relation avec les tests d'écotoxicité ArchiMer
Proniewsk, F.; Lassus, Patrick; Bocquene, Gilles; Miossec, Laurence; Bardouil, Michele.
Dans deux rapports précédents (LASSUS et PRONIEWSKI, 1982 - LE DEAN et Al. , 1983) un certain nombre d'éléments de réponse avaient été apportés quant aux effets d'un pH bas (2,8 à 3,0) en milieu marin, pendant une durée brève, sur la faune et la flore marines.
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Ano: 1984 URL: http://archimer.ifremer.fr/doc/00045/15632/13035.pdf
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Environmental risk assessment: A critical approach of the European TGD in an in situ application ArchiMer
Guerit, Isabelle; Bocquene, Gilles; James, Alice; Thybaud, E; Minier, C.
The aim of this study was to test field relevance of the prospective methodology for the assessment of environmental risk described in the EU technical guidance document (TGD) [European Commission 2003. Technical guidance document in support of Commission Directive 93/67/EEC on Risk assessment for new notified substances and Commission Regulation (EC) No 1488/94 on Risk assessment for existing substances and Commission Directive (EC) 98/8 on biocides, second ed. European Commission, Luxembourg, Part 1, 2 and 3, 760pp.]. To achieve this goal, an environmental risk assessment was performed according to the TGD for two major contaminants, atrazine and diuron, that are present in the Seine River estuary (France) and listed in the EU Water Framework Directive...
Tipo: Text Palavras-chave: Diuron; Atrazine; Estuaries; The Seine River; Technical guidance document; Chemical risk assessment.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4540.pdf
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Pesticide contamination of the coastline of Martinique ArchiMer
Bocquene, Gilles; Franco, Alain.
In January and February 2002, the presence of certain agricultural pesticides throughout the coastline of the Caribbean island of Martinique was investigated. The tropical climate of the French West Indies is suitable for banana production, which requires intensive use of pesticides. An inventory of all pesticides used on the island (compounds and tonnage) was compiled. Surveys and analyses revealed the presence of pesticides in the plumes of seven rivers. The organochlorine chlordecone and metabolites of aldicarb were detected at nearly all of the monitored sites, even though the use of chlordecone has been prohibited since 1993. Two triazines (ametryn and simazine) were also identified. The concentrations of carbamates and triazines detected in the water...
Tipo: Text Palavras-chave: Aldicarb; Chlordecone; Triazines; Pesticides; Contamination; Martinique Island.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-879.pdf
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Toxicité sublétale du tétrachloroéthylène sur la crevette des marais Palaemonetes varians ArchiMer
Lassus, Patrick; Chagot, Dominique; Miossec, Laurence; Bocquene, Gilles; Truquet, Philippe.
Tetrachloroethylene, a chlorinated solvent, is detected in the plume of East-Toulon domestic wastes treatment plant. Its subletal toxicity was tested du ring 31 days in a flow-through system at Pen Bron aquaculture centre, using swampshrimp Palaemonetes varians as test organism. Under expérimental conditions : natural variations in temperature and salinity , normal pH, ammonia and dissolved oxyg~n values, running tetrachloroethylene concentrations (10, 100 and 500 µg.l-1) induced neither mortalities nor any change in molting frequencies of experirnented animaIs. Histology of gills in contaminated animaIs showed several non specific deteriorations which could be observed either in control or assay animaIs. Necrosis levels nevertheless, seem to increase in...
Tipo: Text Palavras-chave: Palaemonetes varians; Crustacés; Tetrachloroethylene; Flux continu; Rejets urbains; Histologie; Nécroses; Palaemonetes varians; Crustacees; Tetrachloroethylene; Flow through system; Urban wastes; Histology; Necrosis.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00156/26707/24782.pdf
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Organochlorinated pesticides, PCBs, dioxins, and PBDEs in grey mullets (Liza ramada) and allis shads (Alosa alosa) from the Vilaine estuary (France) ArchiMer
Bocquene, Gilles; Abarnou, Alain.
This study aimed to compare the contamination levels of various organohalogenated compounds in two migratory fish species in the Vilaine River in western France. Organochlorinated pesticides, polychlorinated biphenyls (PCBs), dioxins (polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs)), and polybrominated diphenyl ethers (PBDEs) were analyzed in two diadromous species from the Vilaine estuary, the grey mullet (Liza ramada)-an amphihaline species, and the allis shad (Alosa alosa)-an anadromous species. Fish were collected in spring 2004 and spring 2005, upstream and downstream of the Arzal Dam. PCB contamination varied from 27 to 200 ng g(-1) dry weight (d.w.). PCDDs/Fs, expressed in toxicity equivalent quantity (TEQ) varied from 0.4 to 2.8 pg g(-1)...
Tipo: Text Palavras-chave: Vilaine estuary; Organohalogenated contaminants; PCBs; DDT; Dioxins; Shad; Grey mullet; Bioaccumulation.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00124/23572/21468.pdf
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A pollution-monitoring pilot study involving contaminant and biomarker measurements in the Seine Estuary, France, using zebra mussels (Dreissena polymorpha) ArchiMer
Minier, Christophe; Abarnou, Alain; Jaouen Madoulet, Agnes; Le Guellec, Anne-marie; Tutundjian, Renaud; Bocquene, Gilles; Leboulenger, François.
Zebra mussel (Dreissena polymorpha) is an invasive species that has proliferated in European and North American rivers and lakes during the last century. In this study, D. polymorpha has been used to provide information on contamination levels and biological effects in the Seine Estuary (France). The bivalves accumulated polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) to a high degree with values reaching 800 ng/g dry weight for PCBs (sum of 20 congeners), and 1,000 ng/g dry weight of PAHs (sum of 14 compounds) in the whole body. These values are among the highest reported of PCBs and, to a lesser extent, of PAHs in other contaminated areas in the world. Toxic equivalent quantities of PCBs and PAHs detected in zebra mussels...
Tipo: Text Palavras-chave: Biomarkers; Multixenobiotic resistance; Polycyclic aromatic hydrocarbons; Polychlorinated biphenyls; Dreissena polymorpha.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2130.pdf
Registros recuperados: 34
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