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| Registros recuperados: 44 | |
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| Apremont, Vincent; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Francois, David; Pradillon, Florence; Corbari, Laure; Zbinden, Magali. |
| Rimicaris chacei Williams and Rona 1986, formerly named as Chorocaris chacei, is a caridean shrimp living in deep-sea hydrothermal ecosystems. This shrimp is endemic to the Mid Atlantic Ridge (MAR) and lives at the periphery of aggregates of its well-known congeneric R. exoculata Williams and Rona 1986. Contrasting with the very dense and mobile clusters formed by R. exoculata, R. chacei lives in small groups of several individuals that are not very mobile. Although devoid of the characteristic hypertrophied cephalothorax of R. exoculata, which harbors the ectosymbionts, a microbial community has also been reported in the cephalothorax of R. chacei. Previous data on morphology, behavior and isotopic values indicate a diet based on a combination of feeding... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00465/57680/59880.pdf |
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| Zeng, Xiang; Birrien, Jean-louis; Fouquet, Yves; Cherkashov, Georgy; Jebbar, Mohamed; Querellou, Joel; Oger, Philippe; Cambon-bonavita, Marie-anne; Xiao, Xiang; Prieur, Daniel. |
| A novel hydrothermal site was discovered in March 2007, on the mid-Atlantic ridge during the cruise 'Serpentine'. At a depth of 4100 m, the site 'Ashadze' is the deepest vent field known so far. Smoker samples were collected with the ROV 'Victor 6000' and processed in the laboratory for the enrichment of anaerobic heterotrophic microorganisms under high-temperature and high-hydrostatic pressure conditions. Strain CH1 was successfully isolated and assigned to the genus Pyrococcus, within the Euryarchaeota lineage within the Archaea domain. This organism grows within a temperature range of 80 to 108 degrees C and a pressure range of 20 to 120 MPa, with optima for 98 degrees C and 52 MPa respectively. Pyrococcus CH1 represents the first obligate piezophilic... |
| Tipo: Text |
Palavras-chave: Thermococcales; Extremophile; Deep sea hydrothermal vent; High hydrostatic pressure; Archaea. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6812.pdf |
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| Sarradin, Pierre-marie; Sarrazin, Jozee; Cadiou, Jean-francois; Olu, Karine; Desbruyeres, Damien; Rigaud, Vincent; Drogou, Jean-francois; Lecornu, Fabrice; Rolin, Jean-francois; Vuillemin, Renaud; Cambon-bonavita, Marie-anne; Sauter, E; Von Juterzenka, K; Boetius, A; Santos, Rs; Colaco, Ana; Pascoal, A; Oliveira, Paulo; Shillito, Bruce; Zal, F; Schultz, A; Taylor, P; Lane, D; Loke, Rob; Du Buf, H; Waldmann, C; Cormack, A; Sanfilippo, L; Masson, M. |
| The general objective of EXOCET/D is to develop, implement and test specific instruments aimed at exploring, describing, quantifying and monitoring biodiversity in deep-sea fragmented habitats as well as at identifying links between community structure and environmental dynamics. Onboard experimental devices will complement the approach, enabling experiments on species physiology. The EXOCET/D working fields include: video and acoustic imagery, in situ analysis of physico-chemical factors, quantitative sampling of macroand micro-organisms, in vivo experiments, integration of multidisciplinary data, implementation on European deep-submersibles and a final phase of technical and scientific validation |
| Tipo: Text |
Palavras-chave: Deep sea; Ecosystem; Imagery; In situ analysis; Faunal sampling; Submersibles; Observatory. |
| Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2007/publication-3600.pdf |
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| Le Bloa, Simon; Durand, Lucile; Cueff-gauchard, Valerie; Le Bars, Josiane; Taupin, Laure; Marteau, Charlotte; Bazire, Alexis; Cambon-bonavita, Marie-anne. |
| Rimicaris exoculata is a caridean shrimp that dominates the fauna at several hydrothermal vent sites of the Mid-Atlantic Ridge. It has two distinct and stable microbial communities. One of these epibiontic bacterial communities is located in the shrimp gut and has a distribution and role that are poorly understood. The second colonizes its enlarged gill chamber and is involved in host nutrition. It is eliminated after each molt, and has colonization processes reminiscent of those of a biofilm. The presence and expression of genes usually involved in quorum sensing (QS) were then studied. At four sites, Rainbow, TAG, Snake Pit and Logatchev, two lux genes were identified in the R. exoculata epibiontic community at different shrimp molt stages and life... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00377/48841/49268.pdf |
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| Roussel, Erwan; Konn, Cecile; Charlou, Jean-luc; Donval, Jean-pierre; Fouquet, Yves; Querellou, Joel; Prieur, Daniel; Cambon-bonavita, Marie-anne. |
| The distribution of Archaea and methanogenic, methanotrophic and sulfate-reducing communities in three Atlantic ultramafic-hosted hydrothermal systems (Rainbow, Ashadze, Lost City) was compared using 16S rRNA gene and functional gene (mcrA, pmoA and dsrA) clone libraries. The overall archaeal community was diverse and heterogeneously distributed between the hydrothermal sites and the types of samples analyzed (seawater, hydrothermal fluid, chimney and sediment). The Lost City hydrothermal field, characterized by high alkaline warm fluids (pH>11; T<95 °C), harbored a singular archaeal diversity mostly composed of unaffiliated Methanosarcinales. The archaeal communities associated with the recently discovered Ashadze 1 site, one of the deepest active... |
| Tipo: Text |
Palavras-chave: Archaea; Hydrothermal vent; Mid-Atlantic Ridge; 16S rRNA gene; Sediment; Ultramafic. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00067/17836/15573.pdf |
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| Cambon-bonavita, Marie-anne; Aubé, Johanne; Cueff-gauchard, Valerie; Reveillaud, Julie. |
| Background Free-living and symbiotic chemosynthetic microbial communities support primary production and higher trophic levels in deep-sea hydrothermal vents. The shrimp Rimicaris exoculata, which dominates animal communities along the Mid-Atlantic Ridge, houses a complex bacterial community in its enlarged cephalothorax. The dominant bacteria present are from the taxonomic groups Campylobacteria, Desulfobulbia (formerly Deltaproteobacteria), Alphaproteobacteria, Gammaproteobacteria, and some recently discovered iron oxyhydroxide-coated Zetaproteobacteria. This epibiotic consortium uses iron, sulfide, methane, and hydrogen as energy sources. Here, we generated shotgun metagenomes from Rimicaris exoculata cephalothoracic epibiotic communities to reconstruct... |
| Tipo: Text |
Palavras-chave: Niche partitioning; Rimicaris; Metagenome-assembled genomes; Zetaproteobacteria; Holobiont; Hydrothermal vents. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00689/80089/83128.pdf |
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| Laming, Sven; Hourdez, Stéphane; Cambon-bonavita, Marie-anne; Pradillon, Florence. |
| The chemosymbiotic gastropod Alviniconcha (Provannidae), first described in 1988, is one of the most emblematic hydrothermal-vent taxa described from the Central Indian Ridge and the Southwest (SW) Pacific. Symbiotic bacteria found in the gill of Alviniconcha are thought to be their principal source of nutrition. In the SW Pacific, species distributions for A. kojimai, A. boucheti – and to a lesser extent A. strummeri – overlap. While Alviniconcha species do not appear to truly co-exist in these highly energetic but spatially limited habitats, certain species regularly co-occur within a single vent field and in rare instances, the same edifice. Past research suggests that SW-Pacific Alviniconcha species might aggregate around fluids with distinct... |
| Tipo: Text |
Palavras-chave: Deep sea; Chemosymbiotic; Periostracum; Gastropod; Habitat partitioning; Computed tomography; Histology; Taxonomy; 3D model. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00628/74048/73459.pdf |
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| Roussel, Erwan; Cambon-bonavita, Marie-anne; Querellou, Joel; Cragg, B; Webster, G; Prieur, D; Parkes, R. |
| Sub-sea-floor sediments may contain two-thirds of Earth's total prokaryotic biomass. However, this has its basis in data extrapolation from ~500-meter to 4-kilometer depths, whereas the deepest documented prokaryotes are from only 842 meters. Here, we provide evidence for low concentrations of living prokaryotic cells in the deepest (1626 meters below the sea floor), oldest (111 million years old), and potentially hottest (~100°C) marine sediments investigated. These Newfoundland margin sediments also have DNA sequences related to thermophilic and/or hyperthermophilic Archaea. These form two unique clusters within Pyrococcus and Thermococcus genera, suggesting unknown, uncultured groups are present in deep, hot, marine sediments (~54° to 100°C). Sequences... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4209.pdf |
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| Burgaud, Gaetan; Arzur, Danielle; Durand, Lucile; Cambon-bonavita, Marie-anne; Barbier, Georges. |
| Investigations of the diversity of culturable yeasts at deep-sea hydrothermal sites have suggested possible interactions with endemic fauna. Samples were collected during various oceanographic cruises at the Mid-Atlantic Ridge, South Pacific Basins and East Pacific Rise. Cultures of 32 isolates, mostly associated with animals, were collected. Phylogenetic analyses of 26S rRNA gene sequences revealed that the yeasts belonged to Ascomycota and Basidiomycota phyla, with the identification of several genera: Rhodotorula, Rhodosporidium, Candida, Debaryomyces and Cryptococcus. Those genera are usually isolated from deep-sea environments. To our knowledge, this is the first report of yeasts associated with deep-sea hydrothermal animals. |
| Tipo: Text |
Palavras-chave: Yeasts; Hydrothermal vents; Fauna; 26S rRNA gene; FISH. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00006/11704/8475.pdf |
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| Guri, Mathieu; Durand, Lucile; Cueff-gauchard, Valerie; Zbinden, Magali; Crassous, Philippe; Shillito, Bruce; Cambon-bonavita, Marie-anne. |
| The caridean shrimp Rimicaris exoculata dominates the fauna at several Mid-Atlantic Ridge hydrothermal vent sites. This shrimp has an enlarged gill chamber, harboring a dense ectosymbiotic community of chemoautotrophic bacteria associated with mineral oxide deposits. Until now, their acquisition is not fully understood. At three hydrothermal vent sites, we analyzed the epibionts diversity at different moult stages and also in the first stages of the shrimp life (eggs, hatched eggs (with larvae) and juveniles). Hatched eggs associated with young larvae were collected for the first time directly from gravid females at the Logachev vent site during the Serpentine cruise. An approach using 16S rRNA clone libraries, scanning and transmission electron... |
| Tipo: Text |
Palavras-chave: Symbiosis; Larvae; Methanotrophic symbiont; Rimicaris exoculata; Transmission pathways. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00072/18318/15997.pdf |
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| Jan, Cyrielle; Petersen, Jillian M.; Werner, Johannes; Teeling, Hanno; Huang, Sixing; Gloeckner, Frank Oliver; Golyshina, Olga V.; Dubilier, Nicole; Golyshin, Peter N.; Jebbar, Mohamed; Cambon-bonavita, Marie-anne. |
| The gill chamber of deep-sea hydrothermal vent shrimp Rimicaris exoculata hosts a dense community of epibiotic bacteria dominated by filamentous Epsilonproteobacteria and Gammaproteobacteria. Using metagenomics on shrimp from the Rainbow hydrothermal vent field, we showed that both epibiont groups have the potential to grow autotrophically and oxidize reduced sulfur compounds or hydrogen with oxygen or nitrate. For carbon fixation, the Epsilonproteobacteria use the reductive tricarboxylic acid cycle, whereas the Gammaproteobacteria use the Calvin–Benson–Bassham cycle. Only the epsilonproteobacterial epibionts had the genes necessary for producing ammonium. This ability likely minimizes direct competition between epibionts and also broadens the spectrum of... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00177/28874/28622.pdf |
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| Cambon-bonavita, Marie-anne; Nadalig, Thierry; Roussel, Erwan; Delage, Eloise; Duperron, Sebastien; Caprais, Jean-claude; Boetius, A.; Sibuet, Myriam. |
| A giant 800-m-diameter pockmark named REGAB was discovered on the Gabon continental margin actively emitting methane at a water depth of 3200 m. The microbial diversity in sediments from four different assemblages of chemosynthetic organisms, Mytilidae, Vesicomyidae, Sibogliniclae and a bacterial mat, was investigated using comparative 16S rRNA gene sequence analysis. Aggregates of anaerobic methanotrophic archaea (ANME-2) and bacteria of the Desulfosarcina/Desulfococcus cluster were found in all four chemosynthetic habitats. Fluorescence in situ hybridization targeting the ANME-2/Desulfosarcina/Desulfococcus aggregates showed their presence few centimeters (3-5cm) below the surface of sediment. 16S rRNA gene sequences from all known marine ANME groups... |
| Tipo: Text |
Palavras-chave: Microbial phylogeny; Faunal assemblage; Cold seep; Chemosynthetic ecosystems; AOM; REGAB. |
| Ano: 2009 |
URL: https://archimer.ifremer.fr/doc/2009/publication-7298.pdf |
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| Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne. |
| In the Guaymas Basin, the presence at a few tens of kilometers of cold seeps and hydrothermal vents coupled with comparable sedimentary settings and depths offer a unique opportunity to assess and compare the microbial community composition of these deep-sea ecosystems. The microbial diversity in sediments from three cold seep and two hydrothermal vent assemblages were investigated using high-throughput 16S rRNA-sequencing. Numerous bacterial and archaeal lineages were detected in both cold seep and hydrothermal vent sediments. Various potential organic matter degraders (e.g., Chloroflexi, Atribacteria, MBG-D) and methane and sulfur cycling related microorganisms (e.g., ANME and methanogenic lineages, sulfate-reducing lineages) were detected in both... |
| Tipo: Text |
Palavras-chave: Microbial diversity; Hydrothermal vents; Cold seeps; Ribotypes; Methane; Extreme environments. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00415/52640/53496.pdf |
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| Bellec, Laure; Cambon-bonavita, Marie-anne; Durand, Lucile; Aube, Johanne; Gayet, Nicolas; Sandulli, Roberto; Brandily, Christophe; Zeppilli, Daniela. |
| Shallow-water hydrothermal vents are widespread, especially in the Mediterranean Sea, owing to the active volcanism of the area. Apart free microbial communities’ investigations, few biological studies have been leaded yet. Investigations of microbial communities associated with Nematoda, an ecologically important group in sediments, can help to improve our overall understanding of these ecosystems. We used a multidisciplinary-approach, based on microscopic observations (scanning electron microscopy: SEM and Fluorescence In Situ Hybridization: FISH) coupled with a molecular diversity analysis using metabarcoding, based on the 16S rRNA gene (V3-V4 region), to characterize the bacterial community of a free-living marine nematode and its environment, the... |
| Tipo: Text |
Palavras-chave: Nematode; Shallow-water hydrothermal vent; Sulfur-oxidizing bacteria; Iron cycle; Zetaproteobacteria. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75544/76449.pdf |
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| Le Bloa, Simon; Boidin-wichlacz, Céline; Cueff-gauchard, Valerie; Rosa, Rafael Diego; Cuvillier-hot, Virginie; Durand, Lucile; Methou, Pierre; Pradillon, Florence; Cambon-bonavita, Marie-anne; Tasiemski, Aurélie. |
| The symbiotic shrimp Rimicaris exoculata dominates the macrofauna inhabiting the active smokers of the deep-sea mid Atlantic ridge vent fields. We investigated the nature of the host mechanisms controlling the vital and highly specialized ectosymbiotic community confined into its cephalothoracic cavity. R. exoculata belongs to the Pleocyemata, crustacean brooding eggs, usually producing Type I crustins. Unexpectedly, a novel anti-Gram-positive type II crustin was molecularly identified in R. exoculata. Re-crustin is mainly produced by the appendages and the inner surfaces of the cephalothoracic cavity, embedding target epibionts. Symbiosis acquisition and regulating mechanisms are still poorly understood. Yet, symbiotic communities were identified at... |
| Tipo: Text |
Palavras-chave: Extreme; Hydrothermal; Symbiosis; Host-microbe interaction; Invertebrate immunity; Crustacean. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75534/76413.pdf |
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| Wery, Nathalie; Lesongeur, Francoise; Pignet, Patricia; Derennes, Veronique; Cambon-bonavita, Marie-anne; Godfroy, Anne; Barbier, Georges. |
| A thermophilic, anaerobic, chemo-organotrophic sulfur-reducing bacterium, designated MV1075(T), was isolated from a deep-sea hydrothermal chimney sample collected on the Mid-Atlantic Ridge. Cells were rod-shaped with a sheath-like outer structure, motile with polar flagella and stained Gramnegative. They appeared singly, in pairs or in short chains. The temperature range for growth was 25-65 degreesC, with an optimum at 55 degreesC. Growth was observed from ph 5 to ph 9, and the optimum ph was around 7. The salinity range for growth was 15-70 g sea salt I-1 (corresponding to 10-45 g NaCl I-1), with an optimum at 30 g I-1 (20 g NaCl I-1). The isolate was able to grow on a broad spectrum of carbohydrates or complex proteinaceous substrates. Sulfur was not... |
| Tipo: Text |
Palavras-chave: Marinitoga camini; Thermotogales; Thermophile; Deep sea hydrothermal vent. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-587.pdf |
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| Bellec, Laure; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Durand, Lucile; Gayet, Nicolas; Zeppilli, Daniela. |
| Deep-sea hydrothermal vent meiofauna have been the focus of recent research and the discovery of an abundant well-adapted free-living marine nematode on the Mid-Atlantic Ridge offers new perspectives on adaptations to the vent environment. Indeed, knowledge concerning biological interactions of microbes and meiofauna in marine extreme environments is scarce, especially for nematodes. In this study, we used microscopic observations [fluorescence in situ hybridization (FISH) and scanning electron microscopy (SEM)] and metabarcoding of 16S rRNA to characterize the bacterial community of the nematode species Oncholaimus dyvae, an overlooked but ecologically important vent organism. Detection of bacteria in the buccal cavity and on the cuticle (SEM) and... |
| Tipo: Text |
Palavras-chave: Sulfur-oxidizing bacteria; Lucky Strike vent field; Meiofauna; Nematode; Endosymbiont. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00457/56846/58676.pdf |
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| Cruaud, Perrine; Vigneron, Adrien; Lucchetti-miganeh, Celine; Ciron, Pierre Emmanuel; Godfroy, Anne; Cambon-bonavita, Marie-anne. |
| Next-generation sequencing (NGS) opens up exciting possibilities for improving our knowledge of environmental microbial diversity, allowing rapid and cost-effective identification of both cultivated and uncultivated microorganisms. However, library preparation, sequencing, and analysis of the results can provide inaccurate representations of the studied community compositions. Therefore, all these steps need to be taken into account carefully. Here we evaluated the effects of DNA extraction methods, targeted 16S rRNA hypervariable regions, and sample origins on the diverse microbes detected by 454 pyrosequencing in marine cold seep and hydrothermal vent sediments. To assign the reads with enough taxonomic precision, we built a database with about 2,500... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00204/31495/29907.pdf |
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| Registros recuperados: 44 | |
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