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| Registros recuperados: 24 | |
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| Tambasco-Talhari,Daniella; Alencar,Maurício Mello de; Paz,Cláudia Cristina Paro de; Cruz,Geraldo Maria da; Rodrigues,Armando de Andrade; Packer,Irineu Umberto; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida. |
| Several studies have investigated the relationship between heterozygosity, genetic distance and production traits. The objective of the present study was to evaluate the influence of the degree of heterozygosity and genetic distance on growth, carcass and reproductive related features in F1 bovine crosses. We tested 10 polymorphic markers in 330 purebred cattle (Nelore, Canchim, Aberdeen Angus and Simental) and 256 crossbred cattle belonging to four crossbred groups. Individual heterozygosities (Hi) and multilocus genetic similarity (Dm) were estimated and used in correlation analysis against individual phenotypic measurements. Significant (p < 0.05) Hi effects occurred for birth weight, 15 to 18 month weight, hot carcass weight and longissimus rib eye... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine crosses; Molecular markers; Heterozygosity; Genetic distance; Production traits. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200007 |
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| Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida. |
| Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005 |
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| Zaros,Lilian Giotto; Bricarello,Patrízia Ana; Amarante,Alessandro Francisco Talamini; Coutinho,Luiz Lehmann. |
| T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cytokine gene expression; Real-time RT-PCR; Cattle; Gastrointestinal nematodes; Bovine cytokines. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012 |
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| Cassoli,Clarissa Sanches da Silva; Jorge,Erika Cirstina; Patrício,Mateus; Alves,Helena Javiel; Almeida,Erik Amazonas de; Ledur,Mônica Corrêa; Coutinho,Luiz Lehmann. |
| Brazilian poultry industry has reached a high level of development in both meat and egg production as a result of constant technological modernization. Further improvements can be achieved through genomics, but before this can be accomplished, a better understanding of gene expression profiles and nucleotide polymorphisms is necessary. Since animal physiology is directly or indirectly controlled by the pituitary and hypothalamus, the aim of the present work was to identify and analyze genes expressed in these tissues in chicken lines with different growth potential. Two pituitary and hypothalamus cDNA libraries from 21 day broiler (TT) and layer (CC) chickens lines were constructed and allowed identification of 3,074 unique sequences and 77 single... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pituitary; Hypothalamus; EST; SNP; Chicken. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600008 |
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| Miyata,Marcelo; Gasparin,Gustavo; Coutinho,Luiz Lehmann; Martinez,Mario Luiz; Machado,Marco Antonio; Silva,Marcos Vinicius G. Barbosa da; Campos,Ana Lucia; Sonstegard,Tad S.; Rosário,Millor Fernandes do; Regitano,Luciana Correia de Almeida. |
| Quantitative trait loci (QTL) mapping in livestock allows the identification of genes that determine the genetic variation affecting traits of economic interest. We analyzed the birth weight and weight at 60 days QTL segregating on bovine chromosome BTA14 in a F2 resource population using genotypes produced from seven microsatellite markers. Phenotypes were derived from 346 F2 progeny produced from crossing Bos indicus Gyr x Holstein Bos taurus F1 parents. Interval analysis to detect QTL for birth weight revealed the presence of a QTL (p < 0.05) at 1 centimorgan (cM) from the centromere with an additive effect of 1.210 ± 0.438 kg. Interval analysis for weight at 60 days revealed the presence of a QTL (p < 0.05) at 0 cM from the centromere with an... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: TA14; Cattle; F2; Growth traits; Molecular markers; QTL. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300011 |
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| Kemenes,Paola Augusta; Regitano,Luciana Correia de Almeida; Rosa,Artur Jordão de Magalhães; Packer,Irineu Umberto; Razook,Alexander George; Figueiredo,Leopoldo Andrade de; Silva,Nirlei Aparecida; Etchegaray,Maria Antônia L.; Coutinho,Luiz Lehmann. |
| The genotypes for k-casein (<FONT FACE="Symbol">k</FONT>-CN), <FONT FACE="Symbol">b</FONT>-lactoglobulin (<FONT FACE="Symbol">b</FONT>-LG) and growth hormone (GH) were determined by polymerase chain reaction (PCR) and restriction enzyme digestion in seven breeds of cattle (Nelore, Gyr, Guzerá, Caracu, Charolais, Canchim and Santa Gertrudis). <FONT FACE="Symbol">k</FONT>-Casein had two alleles with the A allele occurring at a higher frequency in Bos indicus breeds (0.93, 0.92 and 0.91% for Gyr, Guzerá and Nelore, respectively). The <FONT FACE="Symbol">b</FONT>-lactoglobulin locus had two alleles in all of the breeds. European breeds had a higher frequency of the <FONT... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400012 |
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| Lopes,Mário Lúcio; Schmidt,Gilberto Silber; Coutinho,Luiz Lehmann. |
| Chicken embryos were incubated with BrdU, embedded in plastic resin, sectioned and screened immunohistochemically to identify proliferating cells in the neural tube and somites. Fixation in 4% paraformaldehyde for 1 h was essential for detecting specific colorimetric signals of BrdU incorporation into cells during the S phase of the cell cycle. Transverse sections of the neural tube showed that the nuclei of proliferating cells (BrdU positive) had a uniform and centralized distribution, whereas unstained nuclei were found only along the extremities of the neural tube. Transverse sections of differentiated somites showed proliferating cells in the scleratome and dermatome. However, no incorporation of BrdU was observed in myotomic cells, which give rise to... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100028 |
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| Zaros,Lilian Giotto; Coutinho,Luiz Lehmann; Sider,Lúcia Helena; Medeiros,Henrique Rocha de; Neves,Maria Rosalba Moreira das; Benvenuti,Camila Loures; Navarro,Andrine Maria do Carmo; Vieira,Luiz da Silva. |
| Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Control genes; Gastrointestinal nematodes; Ovine; Real-time RT-PCR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300018 |
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| Silva,Fernanda Eliza de Jesus; Guido,Luiza Nicolosi; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Ledur,Mônica Corrêa; Coutinho,Luiz Lehmann; Rosário,Millor Fernandes do. |
| O objetivo deste trabalho foi caracterizar genotipicamente, e construir o mapa de ligação e mapear locos associados a características quantitativas (QTL) de desempenho e carcaça no cromossomo 5 de linhagens brasileiras galinhas. Utilizou-se uma população F2 CTCT, resultante do acasalamento entre machos da linhagem de postura CC e fêmeas da linhagem de corte TT. Um total de 356 animais foi genotipado com 11 marcadores microssatélites. A caracterização genotípica foi realizada pela estimação dos seguintes parâmetros genotípicos: conteúdo de informação polimórfica (0,45-0,69), heterozigosidades observada (0,48-1,00) e esperada (0,48-0,74), e número de alelos por loco (3-5). Empregou-se o mapeamento por intervalo combinado à modelagem fenotípica por modelo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avicultura; Mapeamento por intervalo; Marcadores microssatélites; Locos de características quantitativas. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000300002 |
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| Sousa Junior,Luis Paulo Batista; Meira,Ariana Nascimento; Azevedo,Hymerson Costa; Muniz,Evandro Nevez; Coutinho,Luiz Lehmann; Mourão,Gerson Barreto; Pedrosa,Victor Breno; Pinto,Luís Fernando Batista. |
| Abstract: The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovis aries; Candidate genes; Frequencies; Variants. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102810 |
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| Pinto,Luís Fernando Batista; Packer,Irineu Umberto; Ledur,Mônica Corrêa; Moura,Ana Silvia Alves Meira Tavares; Nones,Kátia; Coutinho,Luiz Lehmann. |
| This study aimed at mapping QTL (quantitative trait loci) using linear combinations of characteristics of economical interest in Gallus gallus. A total of 350 F2 chickens from an initial crossing among males from a broiler line (TT) with females from a layer line (CC) were used. It was conducted a QTL mapping in chromosomes of Gallus gallus (GGA1, GGA3, GGA5, GGA8, GGA11, and GGA13) for 20 performance and carcass traits. For detecting QTL, it was used the likelihood ratio test between a reduced model (including fixed effects of sex, hatch and random effect of infinitesimal genetic value) and a full model (including all the previous effects plus QTL effects). When original characterists were analyzed, that is, before the formation of linear combinations,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broilers; Canonical variables; Chickens; Genome. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001100016 |
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| Tambasco,Daniella Debenedetti; Alencar,Maurício Mello de; Coutinho,Luiz Lehmann; Tambasco,Antonio Junqueira; Tambasco,Marina Debenedetti; Regitano,Luciana Correia de Almeida. |
| No presente trabalho, 180 fêmeas da raça Nelore, provenientes de oito rebanhos, foram analisadas quanto aos marcadores microssatélites TEXAN15, BM1224 e CSFM50 e aos polimorfismos de comprimento de fragmentos de restrição (RFLP) nos locos k-caseína, beta-lactoglobulina e hormônio de crescimento (GH). Com exceção de GH, todos os marcadores foram polimórficos na amostra estudada. Os valores de heterozigosidade, diversidade gênica, conteúdo de informação polimórfica (PIC) e probabilidade de exclusão de paternidade (PE) foram estimados. Os maiores valores de PIC (0,685) e PE (0,521) foram obtidos para o marcador BM1224. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; DNA; Marcador; Microssatélite; Nelore; RFLP. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982000000400014 |
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| Ninov,Kerli; Ledur,Mônica Corrêa; Alves,Helena Javiel; Rosário,Millor Fernandes do; Nones,Kátia; Coutinho,Luiz Lehmann. |
| Leptin, a polypeptide hormone secreted mainly by adipose tissue, plays an important role in feed intake regulation, energy metabolism and reproduction in several species. Its function has been intensively studied in mammals; however, in birds limited information is available. The cDNA sequence for chicken leptin has been reported, and high hepatic expression levels of leptin were associated with fat deposition in selected bird lines. However, controversies still remain concerning to the chicken leptin gene and several authors failed to amplify this gene from genomic DNA or cDNA. In view of this controversy and the importance of this gene, the present study aimed to investigate the leptin gene in a population of birds developed by Embrapa Swine and Poultry... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gallus gallus; Fat deposition; Primer design. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000200016 |
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| Ambo,Marcel; Campos,Raquel de Lello Rocha; Moura,Ana Sílvia Meira Tavares; Boschiero,Clarissa; Rosário,Millor Fernandes do; Ledur,Mônica Corrêa; Nones,Kátia; Coutinho,Luiz Lehmann. |
| A linkage map is essential not only for quantitative trait loci (QTL) mapping, but also for the organization and location of genes along the chromosomes. The present study is part of a project whose major objective is, besides from construction the linkage maps, the whole genome scan for mapping QTL for performance traits in the Brazilian experimental chicken population. Linkage maps of chicken chromosomes 6 to 8, 11 and 13 were constructed based on this population. The population was developed from two generations of crossbreeding between a broiler and a layer line. Fifty-one microsatellite markers were tested, from which 28 were informative: 4, 8, 7, 4 and 5 for chromosomes 6, 7, 8, 11 and 13, respectively. A SNP located in the leptin receptor gene was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gallus gallus; Quantitative trait loci (QTL); Genetic maps; Microsatellite markers; Animal breeding. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500001 |
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| Machado,Alessandro Lima; Meira,Ariana Nascimento; Jucá,Adriana de Farias; Azevedo,Hymerson Costa; Muniz,Evandro Neves; Coutinho,Luiz Lehmann; Mourão,Gerson Barreto; Pedrosa,Victor Breno; Pinto,Luís Fernando Batista. |
| ABSTRACT: Growth hormone (GH), insulin-like growth factor 1 (IGF1), and leptin (LEP) can be candidate genes for association studies because they play vital roles in the metabolism process. Thus, this study aimed to identify variants in these genes associated with body traits in Santa Inês sheep. The following were recorded: body weight at 100 (BW100) and 240 days (BW240), average daily gain (ADG), withers (WH) and croup (CH) heights, body length (BL), thoracic (TG) and leg (LG) girths, thoracic (TW) and croup (CW) widths, body depth (BD), rib eye area (REA), fat thickness (FT), and carcass finishing score (CFS). Single-locus association analysis was performed with 11 variants in IGF1, 18 in LEP, and 16 in GH. Moreover, two haplotypes in IGF1 and one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hormone; Carcass; Growth; Haplotype; Ovine. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000300401 |
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| Ruy,Deborah Clea; Nones,Kátia; Baron,Erica Elias; Ledur,Mônica Corrêa; Melo,Cláudio Manoel Rodrigues de; Ambo,Marcel; Campos,Raquel de Lello Rocha; Coutinho,Luiz Lehmann. |
| Selective genotyping for a certain trait in individuals with extreme phenotypes contributes sufficient information to determine linkage between molecular markers and quantitative trait loci (QTL). In this experiment an F2 population, developed by crossing males from a broiler line with females from a layer line, was employed to detect QTL on chromosomes 3 and 5. Twenty-eight performance and carcass traits were measured in F2 offspring, and phenotypic correlations between traits were calculated. Body weight at 42 days (BW42) presented the greatest positive correlations with most other traits, with correlation between body weights at 35 and 41 days, weight gain between birth and 35, 41 and 42 days, as well as weights of carcass and some body parts superior... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: QTL; Broiler; Selective genotyping; Body weight. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200003 |
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| Registros recuperados: 24 | |
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