SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Roat,Thaisa C.; Nocelli,Roberta C.F.; Cruz-Landim,Carminda da.

The present study analyzed, the influence of the treatment with juvenile hormone on the ultrastructure of Apis mellifera L. workers' venom glands. Newly emerged workers received topical application of 1 µl of juvenile hormone diluted in hexane, in the concentration of 2 µg/µl. Two controls were used; one control received no treatment (group C1) and other received topical application of 1 µl of hexane (group C2). The aspect of the glandular cells, in not treated newly emerged workers, showed that they are not yet secreting actively. Cellular modifications happened according to the worker age and to the glandular area considered. The most active phase of the gland happened from the emergence to the 14th day. At the 25th day the cells had already lost their...

Tegumentary epithelial glands in the abdomen of virgin and physogastric queens of the stingless bee Scaptotrigona postica Latreille (Meliponini: Trigonina)

Palavras-chave: Secretory cycle; Glandular degeneration; Hexane; Bee.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000400007

Abdalla,Fábio C.; Cruz-Landim,Carminda da; Gracioli,Luciana F..

In the eusocial worker bees, portions of the epithelium of the abdominal epidermis may become hypertrophied and glandular, and produce the wax used to build the brood alveoli. In meliponine bees, the presence of similar epithelium was already observed in the queens, but its function is still unknown. Would this epithelium have homologous function in the workers? May the queens display other functions, besides reproduction, related to those considered exclusive of workers? In order to contribute to solve these questions, a histological and ultrastructural study of the tegumentary epithelial glands of the third tergite was carried out in virgins and physogastric queens of Scaptotrigona postica Latreille. The glandular tegumentary epithelium was easily...

Electrophoretic protein pattern and acid phosphatase activity in the midgut extracts of Apis mellifera L. (Hymenoptera: Apidae) during metamorphosis

Palavras-chave: Wax; Meliponine; Histology; Microscopy.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100006

Cavalcante,Vagner M.; Cruz-Landim,Carminda da.

The electrophoretic pattern of proteins and the activity of acid phosphatase were described in the midgut extracts during the post-embryonic stages of Apis mellifera L. in order to stablish a correlation with the metamorphosis events. The results show the greatest variation in proteic electrophoretical bands during prepupal stage and the smallest variation during the brown eyed pupae stage. The acid phosphatase activity was higher during the larval last instar and lower in white eyed pupae. The greatest variety of bands during the prepupal stage coincides with the histolysis of the larval midgut epithelium and the lowest variability in the brown eyed pupa coincides with the end of the midgut differentiation. In another hand, the greatest phosphatase...

Comparative anatomy of the male reproductive internal organs of 51 species of bees

Palavras-chave: Differentiation; Enzymes; Histolysis; Larva; Prepupa; Post-embryonic development; Pupa.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000200007

Ferreira,Amilton; Abdalla,Fábio C.; Kerr,Warwick E.; Cruz-Landim,Carminda da.

The anatomy of the internal organs of the male reproductive apparatus (MRA) of adults and pupae was compared among 51 species of bees, including representatives of six families. Four different types of MRA were found. The type I is present in males of the less derived families (Colletidae, Andrenidae, and Halictidae) and is characterized by three seminiferous tubules per testis, which are almost completely enveloped by the scrotal membrane. The type II is an intermediary between types III and I and is present in Melittidae and Megachilidae, as well as in some Apidae studied, being characterized by post-vesicular deferent ducts outside the scrotal membrane and by three or four seminiferous tubules per testis, except for Apis mellifera L., which has a...

Caracterização das glândulas mandibulares nas diferentes classes de adultos de Scaptotrigona postica Latreille (Hymenoptera: Apidae)

Palavras-chave: Accessory gland; Genitalia; Morphology.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000500005

Gracioli-Vitti,Luciana F.; Abdalla,Fábio C.; Cruz-Landim,Carminda da.

As glândulas mandibulares de operárias recém-emergidas, nutridoras e campeiras, rainhas virgens e fisogástricas, e machos maduros de Scaptotrigona postica Latreille foram estudadas histológica e morfometricamente. Estas glândulas apresentam diferenças histológicas características para cada idade e/ou função do indivíduo. De acordo com a morfometria conclui-se que as glândulas mandibulares possuem um ciclo secretor determinado, no qual a secreção começa a ser produzida nas operárias recém-emergidas, e acumula-se no citoplasma e reservatório da glândula das operárias nutridoras. Nas operárias campeiras o citoplasma encontra-se bastante vacuolizado, o que foi interpretado como resultado da eliminação da secreção. Rainhas fisogástricas apresentaram as maiores...

Ocorrência e morfologia de glândulas tegumentares no abdome de algumas abelhas (Hymenoptera: Apidae): um estudo comparado

Palavras-chave: Anatomia; Histologia; Casta; Sexo; Estado funcional.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000600007

Guerino,Adriane C.; Cruz-Landim,Carminda da.

No presente trabalho foi estudada a ocorrência, distribuição e morfologia de glândulas tegumentares do abdome em Exomalopsis auropilosa Spinola, Centris fuscata Lepeletier, Epicharis flava Friese e Xylocopa (Neoxylocopa) suspecta Moure & Camargo. Os resultados mostraram a ocorrência de glândulas epiteliais (classe I) e unicelulares (classe III) com distribuição dorsal (tergais) e ventral (esternais) sem que se pudesse caracterizar um padrão relacionável com a posição filogenética ou grau de sociabilidade. No entanto, verificou-se uma tendência para maior número de glândulas em espécies com algum grau de sociabilidade como E. auropilosa e X. suspecta.

Ocorrência, morfologia e ultra-estrutura da glândula de Dufour de Scaptotrigona postica Latreille (Hymenoptera: Apidae)

Palavras-chave: Glândula epitelial; Glândula unicelular; Glândula tergal; Glândula esternal.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2003000200010

Abdalla,Fábio C.; Cruz-Landim,Carminda da.

A glândula de Dufour é uma glândula acessória do aparelho reprodutivo feminino das abelhas. Nas abelhas neotropicais sem ferrão, tem sido pouca estudada sob todos os aspectos: morfológico, ontogenético e bioquímico. Na tentativa de colaborar com o conhecimento dessa glândula em abelhas sem ferrão, foi realizado um estudo da sua ocorrência, morfologia e desenvolvimento em Scaptotrigona postica Latreille. Os resultados mostraram que ela se encontra ausente nas operárias, como ocorre em muitas outras espécies desse grupo. Nas rainhas, as células glandulares parecem mais ativas nas virgens, possuindo uma desenvolvida rede de retículo endoplasmático liso tubular, grânulos de secreção e polirribossomos dispersos no citoplasma, além de apresentarem núcleos...

Efeitos da aplicação tópica de hormônio juvenil sobre o desenvolvimento dos ovários de larvas de operárias de Apis mellifera Linnaeus (Hymenoptera, Apidae)

Palavras-chave: Glândula exócrina; Glândula básica; Meliponíneo.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100007

Rev. Bras. entomol.

Antonialli-Junior,William Fernando; Cruz-Landim,Carminda da.

A influência do hormônio juvenil sobre o desenvolvimento do ovário de larvas de operárias de Apis mellifera foi analisada levando em conta a determinação trófica das castas, segundo a qual a alimentação larval é controlada pelas operárias de maneira a promover uma diferenciação de castas controlada pela produção e disponibilidade desse hormônio. A hipótese testada é que a ação do hormônio juvenil seja capaz de proteger ou prevenir a degeneração nos ovários das larvas de operárias. Foi feita aplicação tópica de 1 ml de hormônio dissolvido em hexano na concentração de 1 mg/ml do segundo até o quinto dia de vida larval, e a morfologia dos ovários avaliada nos dias subseqüentes à aplicação até ao sexto dia de vida larval. Como controles foram utilizadas larvas...

Occurrence, morphology and ultrastructure of the Dufour gland in Melipona bicolor Lepeletier (Hymenoptera, Meliponini)

Palavras-chave: Castas; Desenvolvimento pós-embrionário; Número de ovaríolos; Operária.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262009000100025

Rev. Bras. entomol.

Abdalla,Fábio Camargo; Cruz-Landim,Carminda da.

The occurrence, morphology and ultrastructure of the Dufour gland in Melipona bicolor Lepeletier, 1836 are presented. The Dufour gland is not present in workers. In virgin queens the gland cells show characteristics of low activity, which are described in the text. In physogastric queens the gland epithelium is higher and the cells more active than in virgin queens, showing numerous basal plasmic membrane invaginations impregnated by an electrondense material, increased apical invaginations and accumulation of substances that will be released to the gland lumen in the subcuticular space. Therefore, the data show that the Dufour gland is more developed in physogastric than in virgin queens, indicating a possible involvement of the Dufour gland in the...

Ocorrência e morfologia de glândulas tegumentares no abdome de Melissoptila richardiae (Bertoni & Scrottky) (Hymenoptera, Apidae, Eucerini)

Palavras-chave: Dufour gland; Melipona bicolor; Meliponine; Morphology; Ultrastructure.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262004000100002

Rev. Bras. entomol.

Cruz-Landim,Carminda da; Oliveira,Vagner T. Paes de.

Foram estudadas a ocorrência e a morfologia de glândulas tegumentares presentes no abdome de fêmeas de Melissoptila richardiae. Os resultados mostram que nesta espécie, células glandulares da classe III são encontradas de duas formas: isoladas nos tergitos e esternitos III e IV e formando um aglomerado de unidades glandulares bilateralmente, entre os segmentos III e IV, os quais liberam seu produto de secreção em um reservatório originado a partir da membrana intersegmental. Os resultados sugerem que o produto secretado é lipídico e, provavelmente volátil.

Changes in the size of cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) queens and workers in different life phases

Palavras-chave: Esternitos; Glândulas abdominais; Reservatório; Tergitos; Voláteis.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262005000400001

Rev. Bras. Zool.

Poiani,Silvana B.; Cruz-Landim,Carminda da.

The bee species of the Apinae, in addition to the thoracic salivary glands, possess a pair of cephalic glands originating as branches of the excretory duct that crosses the head. These glands are known as cephalic salivary or labial cephalic glands. The degree of development of these glands in newly emerged, nurse and forager workers and virgin and egg-laying queens of Apis mellifera Linnaeus, 1758 and Scaptotrigona postica Latreille, 1807 were evaluated by measuring the secretory alveolar units. The area of the secretory alveoli, measured in total gland preparations, was used to evaluate differences in size. In both species, gland size was found to increase progressively from newly emerged workers to foragers and from virgin to egg-laying queens. A...

Cephalic salivary glands of two species of advanced eusocial bees (Hymenoptera: Apidae): morphology and secretion

Palavras-chave: Bee; Labial gland; Morphometry; Pheromone; Salivary system.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000600018

Rev. Bras. Zool.

Poiani,Silvana B.; Cruz-Landim,Carminda da.

Some adult eusocial bees have a pair of cephalic salivary glands (CSG) in addition to the thoracic labial or salivary gland pairs. This paper deals with variations in morphological features and secretion production of the CSG of females and males of Apis mellifera Linnaeus, 1758 and Scaptotrigona postica Latreille, 1807. The following life stages were studied: newly emerged, nurse, and forager workers; newly emerged and egg-laying queens; and newly emerged and sexually mature males. The histological results showed that the CSG differs between the two species in the following features: while alveoli and duct cells are cuboidal in workers and queens of A. mellifera, they change from cuboidal to flat in S. postica as the workers age. The glands of newly...

Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers

Palavras-chave: Apis mellifera; Histochemical; Labial gland; Lipid; Scaptotrigona postica.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000600021

Rev. Bras. Zool.

Poiani,Silvana Beani; Cruz-Landim,Carminda da.

ABSTRACT Secretory cells of the cephalic salivary glands (CSGs) of eusocial bees produce and accumulate lipid-like secretion in the lumens of their alveoli. Correspondingly, secretory cells present typical ultrastructural features of lipid-compound producers. Previous work on bees has revealed inter-specific differences in the chemical composition of secretion, and the production mechanisms and secretory cycle of secretory cells. In this work a comparative analysis of the mechanisms of lipid storage in the CSGs of Apis mellifera (Linnaeus, 1758) and Scaptotrigona postica (Latreille, 1807) workers was carried out. The ultrastructural location of lipids was ascertained using imidazole-osmium (IO), using individuals in different stages of their life cycles....

Ultrastructure of ovarian follicular epithelium of the amazonian fish Pseudotylosurus microps (Gunther) (Teleostei, Belonidae): I. the follicular cells cycle of development

Palavras-chave: Cytochemistry; Excretory; Imidazole-osmium; Insect; Salivary system.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702016000300901

Cruz-Landim,Carminda da; Cruz-Hofling,Maria Alice da.

The present paper deals with the changes on ultrastructural features of the follicular cells along the growth of the oocyte of Pseudotylosurus microps (Gunther, 1868). The epithelium pass from single squamous to pseudostratified and finally cylindric. Remarkable are the changes in the nuclear shapes, the increasing amount of rough endoplasmic reticulum and the formation of large lipid deposits in the follicular cells by the end of vitellogenic phase. Very peculiar intercellular deposits forms between the perinucleolar and vitellogenic phase of oocyte maturation. Although largely referred to in the specialized literature, our attempts to correlate the follicular cells changes with the formation of the oocyte envelopes or even with an endocrine function are...

Ocorrência e morfologia de glândulas nas pernas de Centris e Epicharis (Hymenoptera, Anthophoridae)

Palavras-chave: Pseudotylosurus microps; Follicular epithelium; Ultrastructure; Teleost.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000100010

Franco,Aldirene Costa; Cruz-Landim,Carminda da.

The presence of glands in insect legs is largely difunded. In bees they are present in solitary and social species. In Centris and Epicharis three different types of glandular structures were found: bag-shaped structures in males femur and tibia, unicelular glandular cells in ali segments of all leg pairs in males and females, but more developed in females, and differentiated epidermis in the basitarsus of both sexes. The tarsal gland present in the distal tarsomere of all legs of males and females are a special type of the bag-shaped structure. The nature of secretion and it use is unknow, but some morphological and stainning properties indicate a lipidic nature.

Note on glands present in meliponinae (Hymenoptera, Apidae) bees legs

Palavras-chave: Hymenoptera; Anthophoridae; Bee; Glands; Legs; Secretion.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000600001

Cruz-Landim,Carminda da; Moraes,Regina Lúcia Morelli Silva de; Salles,Heliana Clara; Reginato,Rejane Daniele.

The present paper reports the presence of glandular structures in legs of some stingless bee species. The glands appear as: the epidermis transformation in a glandular epithelium as in basitarsus, an epithelial sac inside the segment as in the femur of queens or in the last tarsomere, as round glandular cells, scattered or forming groupments. The saculiform gland of femur is present only in queens, the other glands are present in males, queens and workers of the studied species, apparently without any type of polymorphism. This occurrence seems indicate that the function of these glands have not to do with the sociality or specific behavior of castes.

Size differences in the Dufour gland of Apis mellifera Linnaeus (Hymenoptera, Apidae) between and within the female castes

Palavras-chave: Hymenoptera; Stingless bees; Legs; Glands.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000100014

Abdalla,Fábio Camargo; Cruz-Landim,Carminda da.

The Dufour gland is found closely associated with the sting apparatus of all hymenopteran females, playing multiple roles among bees. In Apis mellifera Linnaeus, 1758 the gland is connected to the dorsal vaginal wall and, in queens, it produces egg-marking pheromones. In workers the function of this gland is unknown, except by its capacity to mimic the queen secretion in egg-laying workers. In an attempt to understand the development and to substantiate the present knowledge about the Dufour gland in A. mellifera, a morphometric study of the gland between and within the female castes was made. Glands of workers and queens with different ages and life stages were dissected and measured with an ocular micrometer adapted to a stereoscope. The results showed...

Estudo comparativo da área ocupada pelos corpos pedunculados no cérebro de duas espécies de abelhas (Hymenoptera, Apoidea)

Palavras-chave: Apis mellifera; Dufour gland; Exocrine glands.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000500009

Abdalla,Fábio Camargo; Cruz-Landim,Carminda da.

The present study compare the size of the corpora pedunculata (mushroom bodies) of Exomalopsis aureopilosa a quasi-social specie and Apis mellifera a eusocial specie of bees. The aim was to correlate the developmental degree of such structures with the behavior complexity. The results show that the female specimens of both species have the corpora pedunculata with same relative size. However the area occupied by the neurones cellular bodies (glomeruli) is greater in the workers of A. mellifera. In other way in E. aureopilosa the total size of the corpora pedunculata is larger in females, but the glomeruli area is relatively larger in the male.

Determinação das castas em Scaptotrigona postica (Latreille) (Hymenoptera, Apidae, Meliponini): diferenciação do ovário

Palavras-chave: Hymenoptera; Apoidea; Corpora pedunculata; Glomeruli; Castes; Behavior.

Ano: 1996

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751996000400016

Santos,Thaís da Cruz Alves dos; Cruz-Landim,Carminda da.

Both castes of Scaptotrigolia postica (Latreille, 1804) possess four ovarioles in each ovary. Queen and workers have the same ovarian development during the larvallife, but in lhe late larval stage the queen ovary beco me larger. During pupation a higher rate of cell division is observed in queen ovarioles and a higher rale of cell death in workers. Newly emerged workers have short ovarioles with differenriatcd germarium and vitellarium while queens have very long ovarioles with only germarium. Caste deterrnination in rhis species of bee is trophic, but lhe food does not differ in quality, only in quantiry. The food differences only beco me effecri ve by the end of larval stage when the queen larvae have lhe opportunity of eat more. In this way lhe ovary...