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Registros recuperados: 29 | |
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Techio,Vânia Helena; Davide,Lisete Chamma; Pereira,Antônio Vander; Bearzoti,Eduardo. |
Cytotaxonomic studies were carried out on 26 accessions of Pennisetum spp. originating from the Active Germplasm Bank of Embrapa Gado de Leite-Juiz de Fora, Minas Gerais, Brazil.This study presents cytogenetics and reproductive morphological data for each one of these accessions, which allowed groupings and suggest botanical identifications to be established. The metaphases of the accessions characterized as P. purpureum confirmed the presence of 2n = 28 chromosomes that have already been described for this species. For the hybrids between P. purpureum and P. glaucum and for the hexaploids, resulting from the chromosome duplication of these hybrids, 2n = 21 and 2n = 42 chromosomes were confirmed, respectively. The wild accession identified as P. setosum... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pennisetum; Cytogenetic; Taxonomy; Germplasm; Genetic divergence. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200014 |
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Hallak,Angela Maria Gattás; Davide,Lisete Chamma; Souza,Itamar Ferreira. |
Two experiments were conducted to test the allelopathic effect of sorghum (Sorghum bicolor L.) root exudates on bean (Phaseolus vulgaris L.) cell division. Research was conducted in the greenhouse of the Wistock Agricultural Research Institute of Minas Gerais State (EPAMIG) and in a laboratory of the Federal University of Lavras (UFLA). Sorghum variety BR-601 and bean variety Carioca MG were used. The exudate, called sorgoleone (SGL), was obtained by methylene chloride and acetic acid extraction from sorghum roots seven days after sowing on Petri dishes, and refrigerated until use. Solutions of 0.01, 0.05, 0.10, and 0.15 mM were prepared using Johanson solution as the SGL solvent. Seven-day-old bean seedlings grown in vermiculite in a greenhouse were... |
Tipo: Info:eu-repo/semantics/article |
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Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100018 |
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Santos,Fernanda Motta da Costa; Torres,Giovana Augusta; Techio,Vânia Helena; Pereira,Antônio Vander; Davide,Lisete Chamma. |
Abstract: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pennisetum glaucum; Pennisetum purpureum; In situ hybridization; Intergenomic pairing; Interspecific hybridization. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000900814 |
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Campos,José Marcello Salabert de; Calderano,Clara de Azevedo; Pereira,Antônio Vander; Davide,Lisete Chamma; Viccini,Lyderson Facio; Santos,Marcelo de Oliveira. |
Os objetivos deste trabalho foram estabelecer um protocolo eficiente de embriogênese somática, em híbridos triploides entre capim elefante (Pennisetum purpureum Schumach.) e milheto (P. glaucum (L.) R. Br.), e avaliar por citometria de fluxo a estabilidade genômica das plantas obtidas in vitro. A embriogênese somática e a regeneração das plantas foram estabelecidas a partir de embriões zigóticos maduros de híbridos entre capim elefante e milheto. Foram testados quatro tratamentos com 2,4 ácido diclorofenoxiacético (2,4 D), nas concentrações 0, 1, 2 e 3 mg L-1, para indução de calos embriogênicos, e dois tratamentos com inositol a 1 e 2 g L-1, para regeneração das plantas. Os tratamentos foram dispostos em delineamento inteiramente ao acaso. A combinação... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pennisetum glaucum; Pennisetum purpureum; Cultura de tecidos. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000100006 |
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Ferreira,Kátia; Torres,Giovana Augusta; Carvalho,Isabelle Vilela de; Davide,Lisete Chamma. |
The objective of this work was to compare the meiotic behavior and pollen grain viability of three species of Crotalaria. Slides for meiotic analysis were prepared by the air-drying technique. Pollen grain viability was measured by three staining procedures (Alexander's solution, tetrazolium chloride and fluorescein diacetate) and in vitro germination in a sucrose solution. Eight bivalents were observed, confirming previous reports on populations from other regions of Brazil, as well as from other countries. All species showed abnormal meiotic behavior as follows: in Crotalaria micans, cytomixis and abnormal chromosome pairing in diakinesis; in C. spectabilis, abnormal chromosome pairing in diplotene; in C. zanzibarica, shrunk nuclei in leptotene and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalaria; Cytogenetics; Cytomixis; Meiosis; Pollen grain viability. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200012 |
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Abreu,Juscélio Clemente de; Davide,Lisete Chamma; Pereira,Antônio Vander; Barbosa,Sandro. |
O objetivo deste trabalho foi avaliar métodos de duplicação cromossômica, com uso de agentes antimitóticos e diversos materiais botânicos como explantes dos híbridos entre capim-elefante (Pennisetum purpureum Schum.) e milheto (Pennisetum glaucum (L.) R. Br.). Utilizaram-se soluções de colchicina a 50 mg L-1 e 100 mg L-1, e de ciclohexamida 25 mg L-1:8-hidroxiquinoleína 300 mg L-1 (1:1), aplicadas in vitro em segmentos nodais, e in vivo em plântulas e perfilhos, com diferentes períodos de exposição. O efeito dos antimitóticos foi avaliado por meio da taxa de sobrevivência, do número cromossômico e da presença de anomalias no ciclo celular, em meristemas de raízes das plantas sobreviventes. A colchicina apresentou melhor efeito sobre as plântulas, enquanto... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pennisetum purpureum; Pennisetum glaucum; Análise citogenética; Eliminação cromossômica; Híbrido interespecífico. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001100009 |
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Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Souza Sobrinho,Fausto; Davide,Lisete Chamma. |
Breeding programs for Brachiaria spp. use both intraspecies and interspecies crosses between sexual and apomictic plants in order to obtain new cultivars with the desired characteristics. As there are different ploidy levels both within and between species of this genus, it becomes necessary to evaluate the genotypes used in breeding programs, as a guide to breeders when adopting crossing strategies. In this work, DNA content and chromosome number were determined in order to characterise ploidy levels in Brachiaria spp. genotypes. In the analysis of 15 genotypes, DNA content varied with the ploidy levels (2x, 3x and 4x), and between species and/or taxon. The average DNA content was 1.74 pg (2x) in B. ruziziensis, 3.74 pg (4x) in B. decumbens and 3.52 pg... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Germplasm bank; Cytogenetics; Flow cytometry; Forage. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000100008 |
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Pereira,Luciana Lopes Silva; Marcussi,Silvana; Sátiro,Lívia Cabral; Pereira,Chrystian Araujo; Andrade,Larissa Fonseca; Davide,Lisete Chamma; Santos,Custódio Donizete dos. |
This study was conducted to evaluate the potential induction of genotoxic effects of white bean flour using the Comet assay. The test was conducted with human lymphocytes present in whole blood immediately after collection, by incubation with white bean flour in three concentrations (3.92, 9.52 and 18.18 mg/mL) at 37 ºC for 4 h followed by preparation of slides. Samples were considered positive (above 20% damage) when the damage observed to cellular DNA was higher than the negative control. No genotoxic potential was found at the doses tested. However, it would be premature to suggest absence of risk to human health of DNA damage since the exposure of cells to the extract was restricted to four hours rather than a whole cell cycle. Additionally, further... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotoxicity; White bean flour/genotoxic effects; Comet assay/toxicological analysis of food; Food/toxicological analysis. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100012 |
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Tomé,Lívia Gracielle Oliveira; Silva,Adriano Bortolotti da; Pinto,Cesar Augusto Brasil Pereira; Davide,Lisete Chamma; Pereira,Dalvana Sousa; Carvalho,Carlos Roberto de. |
ABSTRACT: The aim of the present study was to evaluate the effect of the colchicine and oryzalin anti-mitotic substances on the induction of tetraploid plants and foliar anatomy of two diploids clones of Solanum commersonii ssp. Nodal segments of Solanum commersonii subsp. commersonii Dun. and Solanum commersonii subsp. malmeanum Bitt. were treated with colchicine (3.5, 5.0, and 6.5mM; 72h) or oryzalin (10, 30, and 50µM; 24h). After the treatment with anti-mitotic substances, nodal segments were inoculated in the MS culture medium and cultivated in vitro (60 days). After in vitro cultivation, the plants were transferred to vases with the substrate Plantmax(r) and kept in the greenhouse (45 days). Plant ploidy level was assessed by flow cytometry and leaf... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Potato; Crop breeding; Flow citometry; Foliar anatomy. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101973 |
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Mauri,Janaína; Pereira,Daniele Laís; Silva,Gabrielle Avelar; Davide,Lisete Chamma; Techio,Vânia Helena; Souza Sobrinho,Fausto; Pereira,Fabricio José. |
ABSTRACT: The digestibility potential of leaves from forages depends on the amount of nutrition in their tissues, with low lignin deposition in the cell wall, mainly parenchyma and phloem. This research evaluated the leaf structure of different Urochloa genotypes and discussed its potential for evaluating digestibility. The cultivars U. brizantha, U. decumbens, U. ruziziensis and three clones of U. ruziziensis (1, 95 and 97), which are under development in breeding programs, were evaluated. Plants were grown under the recommended culture conditions for the Urochloa species. Plants were cut 60 days after sowing, and leaves were collected at 15 days of regrowth. Leaves were fixed in FAA 70 and further stored in 70% ethanol until being submitted to the usual... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Digestibility; Forage production; Leaf anatomy; Sclerenchyma; Lignin. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000400401 |
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Pereira,Roselaine Cristina; Davide,Lisete Chamma; Techio,Vânia Helena; Timbó,Ana Luiza Oliveira. |
A obtenção artificial de genótipos com duplicação cromossômica em forrageiras busca maximizar características de interesse agronômico, como valor nutricional e produção de forragem, resistência a pragas e doenças, tolerância a estresses abióticos e restauração da fertilidade de híbridos estéreis. Outro objetivo da duplicação cromossômica é a geração de variabilidade genética em espécies apomíticas, por meio da duplicação cromossômica de acessos sexuais igualando a ploidia de modo a permitir a realização de cruzamentos e a obtenção de descendentes férteis. A indução de poliploidia é feita utilizando substâncias antimitóticas, sendo a colchicina a mais amplamente usada em plantas forrageiras. Entretanto, devido a sua toxicidade, outras substâncias como... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Poliploidização; Substâncias antimitóticas; Colchicina; Herbicidas; Cafeína; Explantes. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000700023 |
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Registros recuperados: 29 | |
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